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Transl. Med."],"published-print":{"date-parts":[[2026,5,27]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    is not only a ubiquitous gut commensal but also an opportunistic pathogen responsible for severe intestinal and extraintestinal infections. Shiga toxin\u2013producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    (STEC) poses a notable public health threat, particularly in children, where infections can lead to bloody diarrhea and progress to hemolytic uremic syndrome, a life-threatening condition with long-term complications. Antibiotics are contraindicated in STEC infections because of their potential to induce prophages carrying Shiga toxin (\n                    <jats:italic toggle=\"yes\">stx<\/jats:italic>\n                    ) genes, triggering toxin production. Here, we developed a CRISPR-based antimicrobial strategy using a Cas12 nuclease to selectively eliminate O157 STEC clinical isolates, cleaving more than 99% of\n                    <jats:italic toggle=\"yes\">stx<\/jats:italic>\n                    variants, and prevent toxin release. To enable targeted delivery, we engineered a bacteriophage-derived capsid to specifically transfer a nonreplicative DNA payload to\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    O157, preventing its dissemination. Our therapeutic candidate, EB003, reduced bacterial burden in a murine STEC colonization model. Moreover, EB003 mitigated clinical symptoms, abrogated Stx-mediated toxicity, and accelerated epithelial repair at therapeutically relevant doses in an infant rabbit disease model. These findings demonstrate the potential of CRISPR-based antimicrobials for treating STEC infections and support further clinical development of EB003 as a precision therapeutic against antibiotic-refractory bacterial pathogens.\n                  <\/jats:p>","DOI":"10.1126\/scitranslmed.adw8114","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T17:58:36Z","timestamp":1779904716000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":4,"title":["Treatment of Shiga toxin\u2013producing\n                    <i>E. coli<\/i>\n                    infection by CRISPR-Cas\u2013targeted cleavage of the Shiga toxin gene in animal models"],"prefix":"10.1126","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1097-2928","authenticated-orcid":true,"given":"Matthieu","family":"Galtier","sequence":"first","affiliation":[{"name":"Eligo Bioscience, 75013 Paris, 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