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Transl. Med."],"published-print":{"date-parts":[[2026,6,3]]},"abstract":"<jats:p>\n                    Uncontrolled hemorrhage in trauma, surgical, organ-related, and endoscopic settings, particularly in patients receiving antiplatelet therapy, remains difficult to manage clinically. Here, we introduce a high phosphatidylserine (PS)\u2013exposed procoagulant platelet (hPPL) derivative reprogrammed from isolated platelets via calcium ionophore A23187\u2013induced apoptosis, enriched in surface PS and capable of driving rapid hemostasis. Retaining a protein profile akin to resting platelets, hPPLs robustly promoted platelet activation and aggregation in human- and rat-derived plasma and whole blood in vitro and demonstrated superior hemostatic efficacy compared with clinical thrombin and commercial hemostatic materials [microporous polysaccharide hemispheres (MPH) and FIBRILLAR] in murine liver injury and porcine gastric ulcer bleeding models, even under antiplatelet treatment. Mechanistically, hPPLs up-regulated prostaglandin E synthase (PTGES), thereby increasing prostaglandin E2 (PGE\n                    <jats:sub>2<\/jats:sub>\n                    ) production and its receptor 3 (EP3)\u2013mediated platelet activation, which reinforced PS-mediated clot formation. Our findings identified an apoptosis-driven PTGES-PGE\n                    <jats:sub>2<\/jats:sub>\n                    -EP3 signaling axis that augmented PS-mediated coagulation in murine and porcine hemorrhage models and established the hPPL derivative as a topical hemostatic agent with translational potential for organ-related bleeding and distinct advantages in managing complex endoscopic hemorrhages under both physiological and coagulopathic conditions.\n                  <\/jats:p>","DOI":"10.1126\/scitranslmed.aea0189","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T17:58:11Z","timestamp":1779386291000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Reprogrammed apoptotic platelets drive rapid hemostasis through phosphatidylserine and prostaglandin E2 signaling in preclinical models"],"prefix":"10.1126","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0776-0016","authenticated-orcid":true,"given":"Peina","family":"Wang","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China."},{"name":"Department of Histology and Embryology, College of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China."},{"name":"Laboratory of Molecular Iron Metabolism, Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, Ministry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Science, Hebei Normal University, Shijiazhuang 050024, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5537-6499","authenticated-orcid":true,"given":"Shuailun","family":"Du","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China."},{"name":"College of Materials Science and Opto-Electronics Technology, College of Nanoscience and Engineering, School of Advanced Interdisciplinary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3790-9134","authenticated-orcid":true,"given":"Suying","family":"Wu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China."},{"name":"College of Materials Science and Opto-Electronics Technology, College of Nanoscience and Engineering, School of Advanced Interdisciplinary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6508-264X","authenticated-orcid":true,"given":"Yaqi","family":"Zhai","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Bai","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China."},{"name":"Laboratory of Molecular Iron Metabolism, Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, Ministry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Science, Hebei Normal University, Shijiazhuang 050024, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2078-1178","authenticated-orcid":true,"given":"Ammara","family":"Safdar","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China."},{"name":"College of Materials Science and Opto-Electronics Technology, College of Nanoscience and Engineering, School of Advanced Interdisciplinary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6480-6024","authenticated-orcid":true,"given":"Zhenyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7538-9922","authenticated-orcid":true,"given":"Zefang","family":"Lu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China."},{"name":"College of Materials Science and Opto-Electronics Technology, College of Nanoscience and Engineering, School of Advanced Interdisciplinary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6502-4722","authenticated-orcid":true,"given":"Bozhao","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China."},{"name":"College of Materials Science and Opto-Electronics Technology, College of Nanoscience and Engineering, School of Advanced Interdisciplinary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchang","family":"Song","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Delivery Nanocarriers, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, 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