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Mutagenesis of the lysines in this region resulted in a conditional phenotype in vivo, and the mutant proteins were defective for interactions with transcription factor IIA in vitro. Binding of TFIID to DNA was unaffected. These results suggest that the basic domain of TFIID is important for protein-protein interactions.<\/jats:p>","DOI":"10.1126\/science.1546314","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T23:03:06Z","timestamp":1160089386000},"page":"1130-1132","source":"Crossref","is-referenced-by-count":77,"title":["Transcription Factor IID Mutants Defective for Interaction with Transcription Factor IIA"],"prefix":"10.1126","volume":"255","author":[{"given":"Stephen","family":"Buratowski","sequence":"first","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/0076-6879(87)54076-9","volume":"154","year":"1987","unstructured":"BOEKE, J.D., 5-FLUOROOROTIC ACID AS A SELECTIVE AGENT IN YEAST MOLECULAR-GENETICS, METHODS IN ENZYMOLOGY 154: 164 (1987).","journal-title":"METHODS IN ENZYMOLOGY"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0092-8674(89)90578-3","volume":"56","year":"1989","unstructured":"BURATOWSKI, S, 5 INTERMEDIATE COMPLEXES IN TRANSCRIPTION INITIATION BY RNA POLYMERASE-II, CELL 56: 549 (1989).","journal-title":"CELL"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/334037a0","volume":"334","year":"1988","unstructured":"BURATOWSKI, S, FUNCTION OF A YEAST TATA ELEMENT-BINDING PROTEIN IN A MAMMALIAN TRANSCRIPTION SYSTEM, NATURE 334: 37 (1988).","journal-title":"NATURE"},{"key":"e_1_2_1_4_1","first-page":"7509","volume":"88","year":"1991","unstructured":"BURATOWSKI, S, RNA POLYMERASE-II-ASSOCIATED PROTEINS ARE REQUIRED FOR A DNA CONFORMATION CHANGE IN THE TRANSCRIPTION INITIATION COMPLEX, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 7509 (1991).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_5_1","unstructured":"BURATOWSKI S unpublished data."},{"key":"e_1_2_1_6_1","first-page":"9803","volume":"86","year":"1989","unstructured":"CAVALLINI, B, CLONING OF THE GENE ENCODING THE YEAST PROTEIN BTF1Y, WHICH CAN SUBSTITUTE FOR THE HUMAN TATA BOX-BINDING FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 9803 (1989).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/0092-8674(81)90019-2","volume":"66","year":"1991","unstructured":"DYNLACHT, B.D., ISOLATION OF COACTIVATORS ASSOCIATED WITH THE TATA-BINDING PROTEIN THAT MEDIATE TRANSCRIPTIONAL ACTIVATION, CELL 66: 563 (1991).","journal-title":"CELL"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1002\/j.1460-2075.1984.tb02141.x","volume":"3","year":"1984","unstructured":"EGLY, J.M., IS ACTIN A TRANSCRIPTION INITIATION-FACTOR FOR RNA POLYMERASE-B, EMBO JOURNAL 3: 2363 (1984).","journal-title":"EMBO JOURNAL"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90517-5"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0378-1119(88)90202-8","volume":"70","year":"1988","unstructured":"ELLEDGE, S.J., A FAMILY OF VERSATILE CENTROMERIC VECTORS DESIGNED FOR USE IN THE SECTORING-SHUFFLE MUTAGENESIS ASSAY IN SACCHAROMYCES-CEREVISIAE, GENE 70: 303 (1988).","journal-title":"GENE"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1038\/346291a0","volume":"346","year":"1990","unstructured":"FIKES, J.D., STRIKING CONSERVATION OF TFIID IN SCHIZOSACCHAROMYCES-POMBE AND SACCHAROMYCES-CEREVISIAE, NATURE 346: 291 (1990).","journal-title":"NATURE"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1038\/346390a0","volume":"346","year":"1990","unstructured":"GASCH, A, ARABIDOPSIS-THALIANA CONTAINS 2 GENES FOR TFIID, NATURE 346: 390 (1990).","journal-title":"NATURE"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/0092-8674(89)90515-1","volume":"58","year":"1989","unstructured":"HAHN, S, ISOLATION OF THE GENE ENCODING THE YEAST TATA BINDING-PROTEIN TFIID - 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