{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T10:03:52Z","timestamp":1776247432325,"version":"3.50.1"},"reference-count":24,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5065","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1992,6,26]]},"abstract":"<jats:p>\n            The NH\n            <jats:sub>2<\/jats:sub>\n            -terminus of the signal-anchor sequence of an integral, bitopic protein of the outer mitochondrial membrane was extended both in amino acid length (from 11 to 38 amino acids) and net charge (from +4 to +8)\u2014changes that confer on the NH\n            <jats:sub>2<\/jats:sub>\n            -terminus characteristics of a strong matrix-targeting signal. The protein was inserted into the outer membrane but in an inverted orientation (N\n            <jats:sub>cyto<\/jats:sub>\n            -C\n            <jats:sub>in<\/jats:sub>\n            ). These findings suggest that, in common with other membrane systems, the orientation of a protein in the outer mitochondrial membrane can be determined by a signal that causes retention of the NH\n            <jats:sub>2<\/jats:sub>\n            -terminus on the cytosolic side of the membrane.\n          <\/jats:p>","DOI":"10.1126\/science.1615327","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T23:03:06Z","timestamp":1160089386000},"page":"1815-1817","source":"Crossref","is-referenced-by-count":57,"title":["Reversal of the Orientation of an Integral Protein of the Mitochondrial Outer Membrane"],"prefix":"10.1126","volume":"256","author":[{"given":"Jian-Ming","family":"Li","sequence":"first","affiliation":[{"name":"Department of Biochemistry, McGill University, Montreal, Canada H3G 1Y6.."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon C.","family":"Shore","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, McGill University, Montreal, Canada H3G 1Y6.."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"2899","DOI":"10.1021\/bi00607a031","volume":"17","year":"1978","unstructured":"BAKKER, E.P., ACCUMULATION OF THALLOUS IONS (T1+) AS A MEASURE OF ELECTRICAL POTENTIAL DIFFERENCE ACROSS CYTOPLASMIC MEMBRANE OF BACTERIA, BIOCHEMISTRY 17: 2899 (1978).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_2_1","first-page":"1496","volume":"77","year":"1980","unstructured":"BLOBEL, G, INTRACELLULAR PROTEIN TOPOGENESIS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 77: 1496 (1980).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/0092-8674(90)90378-R","volume":"62","year":"1990","unstructured":"BOYD, D, THE ROLE OF CHARGED AMINO-ACIDS IN THE LOCALIZATION OF SECRETED AND MEMBRANE-PROTEINS, CELL 62: 1031 (1990).","journal-title":"CELL"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1432-1033.1982.tb06766.x","volume":"126","year":"1982","unstructured":"FREITAG, H, BIOSYNTHESIS OF MITOCHONDRIAL PORIN AND INSERTION INTO THE OUTER MITOCHONDRIAL-MEMBRANE OF NEUROSPORA-CRASSA, EUROPEAN JOURNAL OF BIOCHEMISTRY 126: 197 (1982).","journal-title":"EUROPEAN JOURNAL OF BIOCHEMISTRY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1083\/jcb.93.1.103","volume":"93","year":"1982","unstructured":"FUJIKI, Y, POLYPEPTIDE AND PHOSPHOLIPID-COMPOSITION OF THE MEMBRANE OF RAT-LIVER PEROXISOMES - 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