{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:05:03Z","timestamp":1705017903464},"reference-count":8,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"3895","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1969,8,22]]},"abstract":"<jats:p>\n            Choleragenic toxin was separated from vascular permeability factor by ion-exchange chromatography of supernatants of dialyzed peptone cultures of\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            . The choleragenic toxin eluted from columns of QAE-Sephadex with low-ionicity systems is free of permeability factor activity. Further elution of these columns with 0.5\n            <jats:italic>M<\/jats:italic>\n            NaCl removes both the permeability factor and residual choleragenic toxin. When this latter material is chromatographed on columns of carboxymethyl-Sephadex, the permeability factor toxin is eluted by 0.02\n            <jats:italic>M<\/jats:italic>\n            phosphate buffer and is free of choleragenic activity. Therefore, choleragenic and permeability factor activities of the type 2 cholera toxins are different and can be separated by these procedures.\n          <\/jats:p>","DOI":"10.1126\/science.165.3895.808","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T12:14:21Z","timestamp":1160050461000},"page":"808-809","source":"Crossref","is-referenced-by-count":16,"title":["Separation of Type 2 Toxins of\n            <i>Vibrio cholerae<\/i>"],"prefix":"10.1126","volume":"165","author":[{"given":"A. Carter","family":"Lewis","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Tennessee Medical Units, Memphis, Tennessee 38103"}]},{"given":"Bob A.","family":"Freeman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Tennessee Medical Units, Memphis, Tennessee 38103"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1146\/annurev.mi.22.100168.001333","volume":"22","year":"1968","unstructured":"BURROWS, W, CHOLERA TOXINS, ANNUAL REVIEW OF MICROBIOLOGY 22: 245 (1968).","journal-title":"ANNUAL REVIEW OF MICROBIOLOGY"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1128\/jb.96.4.1137-1143.1968","volume":"96","year":"1968","unstructured":"COLEMAN, W.H., CHOLERA TOXINS - PURIFICATION AND PRELIMINARY CHARACTERIZATION OF ILEAL LOOP REACTIVE TYPE 2 TOXIN, JOURNAL OF BACTERIOLOGY 96: 1137 (1968).","journal-title":"JOURNAL OF BACTERIOLOGY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1038\/207614a0","volume":"207","year":"1965","unstructured":"CRAIG, J.P., A PERMEABILITY FACTOR (TOXIN) FOUND IN CHOLERA STOOLS AND CULTURE FILTRATES AND ITS NEUTRALIZATION BY CONVALESCENT CHOLERA SERA, NATURE 207: 614 (1965).","journal-title":"NATURE"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1128\/jb.96.1.126-130.1968","volume":"96","year":"1968","unstructured":"EVANS, D.J., IN VITRO PRODUCTION OF CHOLERAGEN AND VASCULAR PERMEABILITY FACTOR BY VIBRIO CHOLERAE, JOURNAL OF BACTERIOLOGY 96: 126 (1968).","journal-title":"JOURNAL OF BACTERIOLOGY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"440","DOI":"10.4049\/jimmunol.96.3.440","volume":"96","year":"1966","unstructured":"FINKELSTEIN, R.A., PATHOGENESIS OF EXPERIMENTAL CHOLERA - BIOLOGIC ACTIVITIES OF PURIFIED PROCHOLERAGEN A, JOURNAL OF IMMUNOLOGY 96: 440 (1966).","journal-title":"JOURNAL OF IMMUNOLOGY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1093\/infdis\/116.5.606","volume":"116","year":"1966","unstructured":"KASAI, G.J., TITRATION OF CHOLERA TOXIN AND ANTITOXIN IN RABBIT ILEAL LOOP, JOURNAL OF INFECTIOUS DISEASES 116: 606 (1966).","journal-title":"JOURNAL OF INFECTIOUS DISEASES"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1128\/jb.96.4.1443-1445.1968","volume":"96","year":"1968","unstructured":"RICHARDSON, S.H., ISOLATION OF CHOLERA TOXINS BY DEXTRAN SULFATE PRECIPITATION, JOURNAL OF BACTERIOLOGY 96: 1443 (1968).","journal-title":"JOURNAL OF BACTERIOLOGY"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.165.3895.808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T14:15:26Z","timestamp":1704982526000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.165.3895.808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,8,22]]},"references-count":8,"journal-issue":{"issue":"3895","published-print":{"date-parts":[[1969,8,22]]}},"alternative-id":["10.1126\/science.165.3895.808"],"URL":"https:\/\/doi.org\/10.1126\/science.165.3895.808","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1969,8,22]]}}}