{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T13:46:29Z","timestamp":1767275189200},"reference-count":8,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5008","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1991,5,17]]},"abstract":"\n Some strains of\n Escherichia coli<\/jats:italic>\n contain retroelements (retrons) that encode genes for reverse transcriptase and branched, multicopy, single-stranded DNA (msDNA) linked to RNA. However, the origin of retrons is unknown. A P4-like cryptic prophage was found that contains a retroelement (retron Ec73) for msDNA-Ec73 in an\n E. coli<\/jats:italic>\n clinical strain. The entire genome of this prophage, named \u03c6R73, is 12.7 kilobase pairs and is flanked by 29-base pair direct repeats derived from the 3\u2032 end of the selenocystyl transfer RNA gene (\n selC<\/jats:italic>\n ). P2 bacteriophage caused excision of the \u03c6R73 prophage and acted as a helper to package \u03c6R73 DNA into an infectious virion. The newly formed \u03c6R73 closely resembled P4 as a virion and in its lytic growth. Retronphage \u03c6R73 lysogenized a new host strain, reintegrating its genome into the\n selC<\/jats:italic>\n gene of the host chromosome and enabling the newly formed lysogens to produce msDNA-Ec73. Hence, retron Ec73 can be transferred intercellularly as part of the genome of a helper-dependent retronphage.\n <\/jats:p>","DOI":"10.1126\/science.1709758","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T22:10:16Z","timestamp":1160086216000},"page":"969-971","source":"Crossref","is-referenced-by-count":83,"title":["Retronphage \u03c6R73: an\n E. coli<\/i>\n Phage That Contains a Retroelement and Integrates Into a tRNA Gene"],"prefix":"10.1126","volume":"252","author":[{"given":"Sumiko","family":"Inouye","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey at Rutgers, Piscataway, NJ 08854."}]},{"given":"Melvin G.","family":"Sunshine","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Iowa, Iowa City, IA 52242."}]},{"given":"Erich W.","family":"Six","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Iowa, Iowa City, IA 52242."}]},{"given":"Masayori","family":"Inouye","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey at Rutgers, Piscataway, NJ 08854."}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"73","volume":"2","year":"1988","unstructured":"Bertani, L. E., The Baceriophages 2: 73 (1988).","journal-title":"The Baceriophages"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1093\/nar\/7.6.1513","volume":"7","year":"1979","unstructured":"BIRNBOIM, H.C., RAPID ALKALINE EXTRACTION PROCEDURE FOR SCREENING RECOMBINANT PLASMID DNA, NUCLEIC ACIDS RESEARCH 7: 1513 (1979).","journal-title":"NUCLEIC ACIDS RESEARCH"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/0042-6822(73)90462-5","volume":"53","year":"1973","unstructured":"GIBBS, W, SATELLITE BACTERIOPHAGE-P4 - CHARACTERIZATION OF MUTANTS IN 2 ESSENTIAL GENES, VIROLOGY 53: 24 (1973).","journal-title":"VIROLOGY"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/0968-0004(91)90010-S","volume":"16","year":"1991","unstructured":"INOUYE, M, RETROELEMENTS IN BACTERIA, TRENDS IN BIOCHEMICAL SCIENCES 16: 18 (1991).","journal-title":"TRENDS IN BIOCHEMICAL SCIENCES"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"8490","DOI":"10.1016\/S0021-9258(19)38915-X","volume":"265","year":"1990","unstructured":"LAMPSON, B.C., REVERSE-TRANSCRIPTASE FROM ESCHERICHIA-COLI EXISTS AS A COMPLEX WITH MSDNA AND IS ABLE TO SYNTHESIZE DOUBLE-STRANDED DNA, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 8490 (1990).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0042-6822(75)90422-5","volume":"67","year":"1975","unstructured":"SIX, E.W., HELPER DEPENDENCE OF SATELLITE BACTERIOPHAGE-P4 - WHICH GENE FUNCTIONS OF BACTERIOPHAGE-P2 ARE NEEDED BY P4, VIROLOGY 67: 249 (1975).","journal-title":"VIROLOGY"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"4171","DOI":"10.1128\/jb.173.13.4171-4181.1991","volume":"173","year":"1991","unstructured":"Sun, J., Journal of Bacteriology 173: 4171 (1991).","journal-title":"Journal of Bacteriology"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1128\/jvi.45.3.1118-1139.1983","volume":"45","year":"1983","unstructured":"WALKER, J.T., COLIPHAGE-P1 MORPHOGENESIS - ANALYSIS OF MUTANTS BY ELECTRON-MICROSCOPY, JOURNAL OF VIROLOGY 45: 1118 (1983).","journal-title":"JOURNAL OF VIROLOGY"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1709758","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T16:40:46Z","timestamp":1704991246000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1709758"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,5,17]]},"references-count":8,"journal-issue":{"issue":"5008","published-print":{"date-parts":[[1991,5,17]]}},"alternative-id":["10.1126\/science.1709758"],"URL":"https:\/\/doi.org\/10.1126\/science.1709758","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,5,17]]}}}