{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T09:41:51Z","timestamp":1770630111397,"version":"3.49.0"},"reference-count":47,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5023","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1991,8,30]]},"abstract":"<jats:p>\n            In simple eukaryotes, protein kinases regulate mitotic and meiotic cell cycles, the response to polypeptide pheromones, and the initiation of nuclear DNA synthesis. The protein HRR25 from the budding yeast\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            was defined by the mutation\n            <jats:italic>hrr25-1<\/jats:italic>\n            . This mutation resulted in sensitivity to continuous expression of the HO double-strand endonuclease, to methyl methanesulfonate, and to x-irradiation. Homozygotes of\n            <jats:italic>hrr25-1<\/jats:italic>\n            were unable to sporulate and disruption and deletion of\n            <jats:italic>HRR25<\/jats:italic>\n            interfered with mitotic and meiotic cell division. Sequence analysis revealed two distinctive regions in the protein. The NH\n            <jats:sub>2<\/jats:sub>\n            -terminus of HRR25 contains the hallmark features of protein kinases, whereas the COOH-terminus is rich in proline and glutamine. Mutations in\n            <jats:italic>HRR25<\/jats:italic>\n            at conserved residues found in all protein kinases inactivated the gene, and these mutants exhibited the\n            <jats:italic>hrr25<\/jats:italic>\n            null phenotypes. Taken together, the\n            <jats:italic>hrr25<\/jats:italic>\n            mutant phenotypes and the features of the gene product indicate that\n            <jats:italic>HRR25<\/jats:italic>\n            is a distinctive member of the protein kinase superfamily.\n          <\/jats:p>","DOI":"10.1126\/science.1887218","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T22:10:16Z","timestamp":1160086216000},"page":"1031-1034","source":"Crossref","is-referenced-by-count":146,"title":["HRR25, a Putative Protein Kinase from Budding Yeast: Association with Repair of Damaged DNA"],"prefix":"10.1126","volume":"253","author":[{"given":"Merl F.","family":"Hoekstra","sequence":"first","affiliation":[{"name":"Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92186."}]},{"given":"R. Michael","family":"Liskay","sequence":"additional","affiliation":[{"name":"Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06510."}]},{"given":"Alan C.","family":"Ou","sequence":"additional","affiliation":[{"name":"Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92186."}]},{"given":"Anthony J.","family":"DeMaggio","sequence":"additional","affiliation":[{"name":"Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92186."}]},{"given":"David G.","family":"Burbee","sequence":"additional","affiliation":[{"name":"Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92186."}]},{"given":"Fred","family":"Heffron","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Oregon Health Sciences University, Portland, OR 97201."}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/116.4.541"},{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3037703"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2825349"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2402639"},{"key":"e_1_2_1_5_1","first-page":"3101","volume":"9","year":"1989","unstructured":"COLE, G.M., 2 DNA-REPAIR AND RECOMBINATION GENES IN SACCHAROMYCES-CEREVISIAE, RAD52 AND RAD54, ARE INDUCED DURING MEIOSIS, MOLECULAR AND CELLULAR BIOLOGY 9: 3101 (1989).","journal-title":"MOLECULAR AND CELLULAR BIOLOGY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90144-4"},{"key":"e_1_2_1_7_1","first-page":"201","volume":"83","year":"1988","unstructured":"FARNET, C, UCLA SYMPOSIA ON MOLECULAR AND CELLULAR BIOLOGY 83: 201 (1988).","journal-title":"UCLA SYMPOSIA ON MOLECULAR AND CELLULAR BIOLOGY"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.52.1.70-102.1988"},{"key":"e_1_2_1_9_1","first-page":"109","year":"1983","unstructured":"Game, J. 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