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The robust change in emission ratio reveals quantitative heterogeneity in real-time gene expression, enables clonal selection by flow cytometry, and forms a basis for high-throughput screening of pharmaceutical candidate drugs in living mammalian cells.<\/jats:p>","DOI":"10.1126\/science.279.5347.84","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:42:47Z","timestamp":1027762967000},"page":"84-88","source":"Crossref","is-referenced-by-count":624,"title":["Quantitation of Transcription and Clonal Selection of Single Living Cells with \u03b2-Lactamase as Reporter"],"prefix":"10.1126","volume":"279","author":[{"given":"Gregor","family":"Zlokarnik","sequence":"first","affiliation":[{"name":"G. Zlokarnik, P. A. Negulescu, T. E. Knapp, L. Mere, N. Burres, L. Feng, M. Whitney, Aurora Biosciences, 11010 Torreyana Road, San Diego, CA 92121, USA."},{"name":"K. Roemer, University of the Saarland, D-66421 Homburg\/Saar, Germany."},{"name":"R. Y. Tsien, Howard Hughes Medical Institute and Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093\u20130647, USA."}]},{"given":"Paul A.","family":"Negulescu","sequence":"additional","affiliation":[{"name":"G. Zlokarnik, P. A. Negulescu, T. E. Knapp, L. Mere, N. Burres, L. Feng, M. Whitney, Aurora Biosciences, 11010 Torreyana Road, San Diego, CA 92121, USA."},{"name":"K. Roemer, University of the Saarland, D-66421 Homburg\/Saar, Germany."},{"name":"R. Y. Tsien, Howard Hughes Medical Institute and Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093\u20130647, USA."}]},{"given":"Thomas E.","family":"Knapp","sequence":"additional","affiliation":[{"name":"G. Zlokarnik, P. A. Negulescu, T. E. Knapp, L. Mere, N. Burres, L. Feng, M. Whitney, Aurora Biosciences, 11010 Torreyana Road, San Diego, CA 92121, USA."},{"name":"K. Roemer, University of the Saarland, D-66421 Homburg\/Saar, Germany."},{"name":"R. Y. 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Simon for the M 1 receptor gene and S. Ho and G. Crabtree for a plasmid containing a tandem NF-AT\u2013binding site trimer. The work in the Tsien lab was supported by NIH grant NS27177 and the Howard Hughes Medical Institute."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.279.5347.84","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T04:55:39Z","timestamp":1705121739000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.279.5347.84"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1,2]]},"references-count":30,"journal-issue":{"issue":"5347","published-print":{"date-parts":[[1998,1,2]]}},"alternative-id":["10.1126\/science.279.5347.84"],"URL":"https:\/\/doi.org\/10.1126\/science.279.5347.84","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1,2]]}}}