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Transformants contained one or two copies of chromosomally integrated, intact ry1 that were stably inherited in subsequent generations. These transformed flies had wild-type eye color indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene. To demonstrate the generality of this approach, a DNA segment that does not confer a recognizable phenotype on recipients was also transferred into germ line chromosomes.\n          <\/jats:p>","DOI":"10.1126\/science.6289436","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T18:30:32Z","timestamp":1160073032000},"page":"348-353","source":"Crossref","is-referenced-by-count":2409,"title":["Genetic Transformation of\n            <i>Drosophila<\/i>\n            with Transposable Element Vectors"],"prefix":"10.1126","volume":"218","author":[{"given":"Gerald M.","family":"Rubin","sequence":"first","affiliation":[{"name":"Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan C.","family":"Spradling","sequence":"additional","affiliation":[{"name":"Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"5399","volume":"77","year":"1980","unstructured":"ANDERSON, W.F., REPLICATION AND EXPRESSION OF THYMIDINE KINASE AND HUMAN GLOBIN GENES MICRO-INJECTED INTO MOUSE FIBROBLASTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 77: 5399 (1980).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES"},{"key":"e_1_2_1_2_1","first-page":"1590","volume":"74","year":"1977","unstructured":"BACCHETTI, S, TRANSFER OF GENE FOR THYMIDINE KINASE TO THYMIDINE KINASE-DEFICIENT HUMAN CELLS BY PURIFIED HERPES-SIMPLEX VIRAL-DNA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 74: 1590 (1977).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.ge.12.120178.002315","volume":"12","year":"1978","unstructured":"BAKER, W.K., GENETIC FRAMEWORK FOR DROSOPHILA DEVELOPMENT, ANNUAL REVIEW OF GENETICS 12: 451 (1978).","journal-title":"ANNUAL REVIEW OF GENETICS"},{"key":"e_1_2_1_4_1","unstructured":"BENDER W COMMUNICATION."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a104022"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0092-8674(81)90376-7","volume":"27","year":"1981","unstructured":"BRINSTER, R.L., SOMATIC EXPRESSION OF HERPES THYMIDINE KINASE IN MICE FOLLOWING INJECTION OF A FUSION GENE INTO EGGS, CELL 27: 223 (1981).","journal-title":"CELL"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/0092-8674(80)90358-X","volume":"22","year":"1980","unstructured":"CAPECCHI, M.R., HIGH-EFFICIENCY TRANSFORMATION BY DIRECT MICRO-INJECTION OF DNA INTO CULTURED MAMMALIAN-CELLS, CELL 22: 479 (1980).","journal-title":"CELL"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/294092a0","volume":"294","year":"1981","unstructured":"CONSTANTINI, F, NATURE 294: 92 (1981).","journal-title":"NATURE"},{"key":"e_1_2_1_9_1","first-page":"2268","volume":"79","year":"1982","unstructured":"DOEHMER, J, INTRODUCTION OF RAT GROWTH-HORMONE GENE INTO MOUSE FIBROBLASTS VIA A RETROVIRAL DNA VECTOR - EXPRESSION AND REGULATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 79: 2268 (1982).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES"},{"key":"e_1_2_1_10_1","unstructured":"ENGELS W.R. unpublished data."},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1146\/annurev.ge.10.120176.001233","volume":"10","year":"1976","unstructured":"GEHRING, W.J., DEVELOPMENTAL GENETICS OF DROSOPHILA, ANNUAL REVIEW OF GENETICS 10: 209 (1976).","journal-title":"ANNUAL REVIEW OF GENETICS"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/0092-8674(76)90133-1","volume":"9","year":"1976","unstructured":"Goff, S. 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