{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:06:25Z","timestamp":1770480385374,"version":"3.49.0"},"reference-count":38,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4635","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1984,2,3]]},"abstract":"<jats:p>\n            The nucleotide sequences of the six regions within the normal human cellular locus (c-\n            <jats:italic>sis<\/jats:italic>\n            ) that correspond to the entire transforming region of the simian sarcoma virus (SSV) genome (v-\n            <jats:italic>sis<\/jats:italic>\n            ) were determined. The regions are bounded by acceptor and donor splice sites and, except for region 6, resemble exons. Region 6 lacks a 3\u2032 donor splice site and terminates -5 base pairs from the 3\u2032 v-\n            <jats:italic>sis<\/jats:italic>\n            -helper-viral junction. This is consistent with a model proposing that SSV was generated by recombination between proviral DNA of simian sarcoma associated virus and proto-\n            <jats:italic>sis<\/jats:italic>\n            and that introns were spliced out subsequently from a fused viral-\n            <jats:italic>sis<\/jats:italic>\n            messenger RNA. This also suggests that the 3\u2032 recombination occurred within an exon of the woolly monkey (\n            <jats:italic>Lagothrix<\/jats:italic>\n            ) genome. The open reading frames predicting the v-\n            <jats:italic>sis<\/jats:italic>\n            and c-\n            <jats:italic>sis<\/jats:italic>\n            gene products coincide with the stop codon of c-\n            <jats:italic>sis<\/jats:italic>\n            located 123 nucleotides into the fifth region of homology. The overall nucleotide homology was 91 percent with substitutions mainly in the third codon positions within the open reading frame and with greatest divergence within the untranslated 3\u2032 portion of the sequences. The predicted protein products for v-\n            <jats:italic>sis<\/jats:italic>\n            and c-\n            <jats:italic>sis<\/jats:italic>\n            are 93 percent homologous. The predicted c-\n            <jats:italic>sis<\/jats:italic>\n            gene product is identical in 31 of 31 amino acids to one of the published sequences of platelet-derived growth factor. Thus, c-\n            <jats:italic>sis<\/jats:italic>\n            encodes one chain of human platelet-derived growth factor.\n          <\/jats:p>","DOI":"10.1126\/science.6318322","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T19:41:59Z","timestamp":1160077319000},"page":"487-491","source":"Crossref","is-referenced-by-count":129,"title":["Human-Proto-Oncogene Nucleotide Sequences Corresponding to the Transforming Region of Simian Sarcoma Virus"],"prefix":"10.1126","volume":"223","author":[{"given":"Steven F.","family":"Josephs","sequence":"first","affiliation":[{"name":"Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee","family":"Ratner","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flossie","family":"Wong-Staal","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"1809","volume":"76","year":"1979","unstructured":"ANTONIADES, H.N., PURIFICATION OF HUMAN PLATELET-DERIVED GROWTH-FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 1809 (1979).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6844921"},{"key":"e_1_2_1_3_1","first-page":"496","volume":"309","year":"1983","unstructured":"AURIAS, A, CHROMOSOMAL TRANSLOCATIONS IN EWINGS-SARCOMA, NEW ENGLAND JOURNAL OF MEDICINE 309: 496 (1983).","journal-title":"NEW ENGLAND JOURNAL OF MEDICINE"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/0092-8674(80)90305-0","volume":"20","year":"1980","unstructured":"CALOS, M.P., TRANSPOSABLE ELEMENTS, CELL 20: 579 (1980).","journal-title":"CELL"},{"key":"e_1_2_1_5_1","unstructured":"CLARKE M.F. unpublished data."},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1099\/0022-1317-42-1-1","volume":"42","year":"1979","unstructured":"COFFIN, J.M., STRUCTURE, REPLICATION, AND RECOMBINATION OF RETROVIRUS GENOMES - 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