{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:29:17Z","timestamp":1758396557514},"reference-count":14,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4605","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1983,7]]},"abstract":"<jats:p>\n            Oligonucleotide-directed site-specific mutagenesis was applied to alter the cleavage site in the signal peptide of the major outer membrane lipoprotein of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . Replacing the glycine residue at the cleavage site with an alanine residue did not affect the processing of the signal peptide. However, when the same cleavage site was constructed by the deletion of the glycine residue, the signal peptide was no longer cleaved. These results indicate that stringent structural integrity at the cleavage site in the lipoprotein signal sequence is required for correct processing of prolipoprotein.\n          <\/jats:p>","DOI":"10.1126\/science.6344218","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T18:37:18Z","timestamp":1160073438000},"page":"59-61","source":"Crossref","is-referenced-by-count":50,"title":["Requirement for Signal Peptide Cleavage of\n            <i>Escherichia coli<\/i>\n            Prolipoprotein"],"prefix":"10.1126","volume":"221","author":[{"given":"Sumiko","family":"Inouye","sequence":"first","affiliation":[{"name":"Department of Biochemistry, State University of New York, Stony Brook 11794"}]},{"given":"Chian-Pin Sherman","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Biology, City of Hope Research Institute, Duarte, California 91010"}]},{"given":"Keiichi","family":"Itakura","sequence":"additional","affiliation":[{"name":"Department of Biology, City of Hope Research Institute, Duarte, California 91010"}]},{"given":"Masayori","family":"Inouye","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, State University of New York, Stony Brook 11794"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1128\/jb.115.3.717-722.1973","volume":"115","year":"1973","unstructured":"FILIP, C, SOLUBILIZATION OF CYTOPLASMIC MEMBRANE OF ESCHERICHIA-COLI BY IONIC DETERGENT SODIUM-LAURYL SARCOSINATE, JOURNAL OF BACTERIOLOGY 115: 717 (1973).","journal-title":"JOURNAL OF BACTERIOLOGY"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.3109\/10409238009105465","volume":"7","year":"1980","unstructured":"INOUYE, M, SECRETION AND MEMBRANE LOCALIZATION OF PROTEINS IN ESCHERICHIA-COLI, CRC CRITICAL REVIEWS IN BIOCHEMISTRY 7: 339 (1980).","journal-title":"CRC CRITICAL REVIEWS IN BIOCHEMISTRY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1002\/j.1460-2075.1983.tb01386.x","volume":"2","year":"1983","unstructured":"INOUYE, S, PROLIPOPROTEIN SIGNAL PEPTIDASE OF ESCHERICHIA-COLI REQUIRES A CYSTEINE RESIDUE AT THE CLEAVAGE SITE, EMBO JOURNAL 2: 87 (1983).","journal-title":"EMBO JOURNAL"},{"key":"e_1_2_1_4_1","first-page":"3438","volume":"79","year":"1982","unstructured":"INOUYE, S, ROLE OF POSITIVE CHARGE ON THE AMINO-TERMINAL REGION OF THE SIGNAL PEPTIDE IN PROTEIN SECRETION ACROSS THE MEMBRANE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 79: 3438 (1982).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES"},{"key":"e_1_2_1_5_1","first-page":"1004","volume":"74","year":"1977","unstructured":"INOUYE, S, AMINO-ACID SEQUENCE FOR PEPTIDE EXTENSION ON PRO-LIPOPROTEIN OF ESCHERICHIA-COLI OUTER MEMBRANE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 74: 1004 (1977).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_6_1","unstructured":"INOUYE S unpublished data."},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1432-1033.1983.tb07112.x","volume":"130","year":"1983","unstructured":"Inukai, M., European Journal of Biochemistry 130: 27 (1983).","journal-title":"European Journal of Biochemistry"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.7164\/antibiotics.31.1203","volume":"31","year":"1978","unstructured":"INUKAI, M, MECHANISM OF ACTION OF GLOBOMYCIN, JOURNAL OF ANTIBIOTICS 31: 1203 (1978).","journal-title":"JOURNAL OF ANTIBIOTICS"},{"key":"e_1_2_1_9_1","first-page":"4891","volume":"75","year":"1978","unstructured":"LIN, JJC, ESCHERICHIA-COLI MUTANT WITH AN AMINO-ACID ALTERATION WITHIN SIGNAL SEQUENCE OF OUTER MEMBRANE PROLIPOPROTEIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 4891 (1978).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.2.560"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1002\/j.1460-2075.1982.tb01244.x","volume":"1","year":"1982","unstructured":"NAKAMURA, K, CONSTRUCTION OF VERSATILE EXPRESSION CLONING VEHICLES USING THE LIPOPROTEIN GENE OF ESCHERICHIA-COLI, EMBO JOURNAL 1: 771 (1982).","journal-title":"EMBO JOURNAL"},{"key":"e_1_2_1_12_1","first-page":"289","volume":"1","year":"1982","unstructured":"NAKAMURA, K, JOURNAL OF MOLECULAR AND APPLIED GENETICS 1: 289 (1982).","journal-title":"JOURNAL OF MOLECULAR AND APPLIED GENETICS"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"7141","DOI":"10.1016\/S0021-9258(18)32343-3","volume":"258","year":"1983","unstructured":"Vlasuk, G. P., Journal of Biological Chemistry 258: 7141 (1983).","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1128\/jb.152.3.1163-1168.1982","volume":"152","year":"1982","unstructured":"YAMAGATA, H, TEMPERATURE-SENSITIVE PROCESSING OF OUTER-MEMBRANE LIPOPROTEIN IN AN ESCHERICHIA-COLI MUTANT, JOURNAL OF BACTERIOLOGY 152: 1163 (1982).","journal-title":"JOURNAL OF BACTERIOLOGY"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.6344218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,14]],"date-time":"2024-01-14T22:32:58Z","timestamp":1705271578000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.6344218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,7]]},"references-count":14,"journal-issue":{"issue":"4605","published-print":{"date-parts":[[1983,7]]}},"alternative-id":["10.1126\/science.6344218"],"URL":"https:\/\/doi.org\/10.1126\/science.6344218","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,7]]}}}