{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T10:45:05Z","timestamp":1774867505359,"version":"3.50.1"},"reference-count":11,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4345","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1978,6,2]]},"abstract":"<jats:p>\n            A facile method is described for making magnetic microspheres that bind specifically to cell surfaces, in order to separate cells magnetophoretically. Control over the sizes of the spheres is effected by using their magnetic cores as part of a redox polymerization system. The use of the microspheres is demonstrated with a separation involving C-1300 neuroblastoma cells, 10 percent of which express the ganglioside G\n            <jats:sub>M1<\/jats:sub>\n            in their membranes. The G\n            <jats:sub>M1<\/jats:sub>\n            -containing cells were separated with better than 99 percent purity, while the deficient cells were obtained at least 98 percent pure. The separation, which was carried out under sterile conditions, required only 6 minutes.\n          <\/jats:p>","DOI":"10.1126\/science.653356","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T16:35:25Z","timestamp":1160066125000},"page":"1074-1076","source":"Crossref","is-referenced-by-count":91,"title":["Magnetic Microspheres Prepared by Redox Polymerization Used in a Cell Separation Based on Gangliosides"],"prefix":"10.1126","volume":"200","author":[{"given":"Paul L.","family":"Kronick","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham LeM.","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00742a031"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0006-8993(76)91011-8","volume":"114","year":"1976","unstructured":"DEBAECQUE, C, GANGLIOSIDE LOCALIZATION IN CEREBELLAR CORTEX - IMMUNOPEROXIDASE STUDY WITH ANTIBODY TO GM1 GANGLIOSIDE, BRAIN RESEARCH 114: 117 (1976).","journal-title":"BRAIN RESEARCH"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.185697"},{"key":"e_1_2_1_4_1","first-page":"285","volume":"76","year":"1974","unstructured":"GRAHAM, D.I., UNDIFFERENTIATED AND EXTENDED FORMS OF C1300 MURINE NEUROBLASTOMA - ULTRASTRUCTURAL STUDY AND DETECTION OF CONCANAVALIN-A BINDING-SITES ON PLASMA-MEMBRANE, AMERICAN JOURNAL OF PATHOLOGY 76: 285 (1974).","journal-title":"AMERICAN JOURNAL OF PATHOLOGY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1177\/14.4.291","volume":"14","year":"1966","unstructured":"GRAHAM, R.C., EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN PROXIMAL TUBULES OF MOUSE KIDNEY - ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 14: 291 (1966).","journal-title":"JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY"},{"key":"e_1_2_1_6_1","first-page":"55","volume":"417","year":"1975","unstructured":"HAKAMORI, S.I., BIOCHIM BIOPHYS ACTA 417: 55 (1975).","journal-title":"BIOCHIM BIOPHYS ACTA"},{"key":"e_1_2_1_7_1","first-page":"4224","volume":"71","year":"1974","unstructured":"HOLLENBERG, M.D., CHOLERA TOXIN AND CELL-GROWTH - ROLE OF MEMBRANE GANGLIOSIDES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 4224 (1974).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.959818"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/268437a0","volume":"268","year":"1977","unstructured":"MOLDAY, R.S., APPLICATION OF MAGNETIC MICROSPHERES IN LABELING AND SEPARATION OF CELLS, NATURE 268: 437 (1977).","journal-title":"NATURE"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1084\/jem.143.4.822","volume":"143","year":"1976","unstructured":"STEINDOUGLAS, K.E., GANGLIOSIDES AS MARKERS FOR MURINE LYMPHOCYTE SUBPOPULATIONS, JOURNAL OF EXPERIMENTAL MEDICINE 143: 822 (1976).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0021-9258(19)44287-7","volume":"248","year":"1973","unstructured":"YOGEESWARAN, G, GLYCOSPHINGOLIPIDS OF CLONAL LINES OF MOUSE NEUROBLASTOMA AND NEUROBLASTOMA X L CELL HYBRIDS, JOURNAL OF BIOLOGICAL CHEMISTRY 248: 1231 (1973).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.653356","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,14]],"date-time":"2024-01-14T23:24:20Z","timestamp":1705274660000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.653356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,6,2]]},"references-count":11,"journal-issue":{"issue":"4345","published-print":{"date-parts":[[1978,6,2]]}},"alternative-id":["10.1126\/science.653356"],"URL":"https:\/\/doi.org\/10.1126\/science.653356","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,6,2]]}}}