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Upon recognition of the specific nucleic acid molecule the ends of the probes were joined through the action of a ligase, creating circular DNA molecules catenated to the target sequence. These probes thus provide highly specific detection with minimal background.<\/jats:p>","DOI":"10.1126\/science.7522346","type":"journal-article","created":{"date-parts":[[2006,10,6]],"date-time":"2006-10-06T00:01:25Z","timestamp":1160092885000},"page":"2085-2088","source":"Crossref","is-referenced-by-count":634,"title":["Padlock Probes: Circularizing Oligonucleotides for Localized DNA Detection"],"prefix":"10.1126","volume":"265","author":[{"given":"Mats","family":"Nilsson","sequence":"first","affiliation":[{"name":"The Beijer Laboratory, Department of Medical Genetics, S-75123 Uppsala, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Malmgren","sequence":"additional","affiliation":[{"name":"The Beijer Laboratory, Department of Medical Genetics, S-75123 Uppsala, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Samiotaki","sequence":"additional","affiliation":[{"name":"The Beijer Laboratory, Department of Medical Genetics, S-75123 Uppsala, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Kwiatkowski","sequence":"additional","affiliation":[{"name":"The Beijer Laboratory, Department of Medical Genetics, S-75123 Uppsala, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhanu P.","family":"Chowdhary","sequence":"additional","affiliation":[{"name":"Swedish University of Agricultural Sciences, S-750 07 Uppsala, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf","family":"Landegren","sequence":"additional","affiliation":[{"name":"The Beijer Laboratory, Department of Medical Genetics, S-75123 Uppsala, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"8723","DOI":"10.1093\/nar\/16.17.8723","volume":"16","year":"1988","unstructured":"ALVES, A.M., DOT BLOT DETECTION OF POINT MUTATIONS WITH ADJACENTLY HYBRIDIZING SYNTHETIC OLIGONUCLEOTIDE PROBES, NUCLEIC ACIDS RESEARCH 16: 8723 (1988).","journal-title":"NUCLEIC ACIDS RESEARCH"},{"key":"e_1_2_1_2_1","first-page":"784","volume":"46","year":"1990","unstructured":"BALDINI, A, A HUMAN ALPHA-SATELLITE DNA SUBSET SPECIFIC FOR CHROMOSOME-12, AMERICAN JOURNAL OF HUMAN GENETICS 46: 784 (1990).","journal-title":"AMERICAN JOURNAL OF HUMAN GENETICS"},{"key":"e_1_2_1_3_1","first-page":"189","volume":"88","year":"1991","unstructured":"BARANY, F, GENETIC-DISEASE DETECTION AND DNA AMPLIFICATION USING CLONED THERMOSTABLE LIGASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 189 (1991).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"5403","DOI":"10.1093\/nar\/21.23.5403","volume":"21","year":"1993","unstructured":"DOLINNAYA, N.G., OLIGONUCLEOTIDE CIRCULARIZATION BY TEMPLATE-DIRECTED CHEMICAL LIGATION, NUCLEIC ACIDS RESEARCH 21: 5403 (1993).","journal-title":"NUCLEIC ACIDS RESEARCH"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/0076-6879(79)68006-0","volume":"68","year":"1979","unstructured":"HIGGINS, N.P., METHODS IN ENZYMOLOGY 68: 50 (1979).","journal-title":"METHODS IN ENZYMOLOGY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/S0040-4039(00)60572-5","volume":"34","year":"1993","unstructured":"JASCHKE, A, AUTOMATED INCORPORATION OF POLYETHYLENE-GLYCOL INTO SYNTHETIC OLIGONUCLEOTIDES, TETRAHEDRON LETTERS 34: 301 (1993).","journal-title":"TETRAHEDRON LETTERS"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3413476"},{"key":"e_1_2_1_8_1","unstructured":"Maniatis T. 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