{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:46:17Z","timestamp":1769215577161,"version":"3.49.0"},"reference-count":25,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5653","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,12,19]]},"abstract":"<jats:p>\n            Calorie restriction (CR) slows aging in numerous species. In the yeast\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            , this effect requires Sir2, a conserved NAD\n            <jats:sup>+<\/jats:sup>\n            -dependent deacetylase. We report that CR reduces nuclear NAD\n            <jats:sup>+<\/jats:sup>\n            levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD\n            <jats:sup>+<\/jats:sup>\n            :NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.\n          <\/jats:p>","DOI":"10.1126\/science.1088697","type":"journal-article","created":{"date-parts":[[2003,11,11]],"date-time":"2003-11-11T01:14:32Z","timestamp":1068513272000},"page":"2124-2126","source":"Crossref","is-referenced-by-count":127,"title":["Yeast Life-Span Extension by Calorie Restriction Is Independent of NAD Fluctuation"],"prefix":"10.1126","volume":"302","author":[{"given":"Rozalyn M.","family":"Anderson","sequence":"first","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]},{"given":"Magda","family":"Latorre-Esteves","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]},{"given":"Ana Rute","family":"Neves","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]},{"given":"Siva","family":"Lavu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]},{"given":"Oliver","family":"Medvedik","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]},{"given":"Christopher","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]},{"given":"Konrad T.","family":"Howitz","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]},{"given":"Helena","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]},{"given":"David A.","family":"Sinclair","sequence":"additional","affiliation":[{"name":"Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA."},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal."},{"name":"Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA."}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5487.2126"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.22.8056-8066.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0242fje"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2000_4015"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.12.6658"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/35001622"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.110148297"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01578"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4279"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1082358"},{"key":"e_1_3_2_12_2","first-page":"28088","volume":"13","year":"2002","unstructured":"A. R. Neves et al., J. Biol. Chem.13, 28088 (2002).","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069300"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.2.648-655.1999"},{"key":"e_1_3_2_15_2","unstructured":"Materials and methods are available as supporting material on Science Online."},{"key":"e_1_3_2_16_2","unstructured":"R. M. Anderson et al. unpublished data."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(03)00006-1"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111773200"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/160.3.877"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi011085z"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.66.3.652"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43354-0"},{"key":"e_1_3_2_23_2","unstructured":"H. Sies Metabolic Compartmentation (Academic Press London 1982)."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00226-0"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007164200"},{"key":"e_1_3_2_26_2","unstructured":"We are grateful to D. Moazed P. Frey M. Agosto R. Veech J. Foster (pFW38 \u2013 46) L. Guarente (pADH-HAP4) and B. Forrester (NSY64) for advice and\/or reagents. Synthesis of labeled nicotinic acid was carried out by R. Ventura and C. Maycock. This work was supported by the National Institute on Aging and the Harvard-Armenise Foundation. D.S. is an Ellison Medical Research Foundation Special Fellow. R.A. is supported by a John Taplan Postdoctoral Fellowship O.M. by the American Federation of Aging Research."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1088697","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T02:12:40Z","timestamp":1704852760000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1088697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12,19]]},"references-count":25,"journal-issue":{"issue":"5653","published-print":{"date-parts":[[2003,12,19]]}},"alternative-id":["10.1126\/science.1088697"],"URL":"https:\/\/doi.org\/10.1126\/science.1088697","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,12,19]]}}}