{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:26:31Z","timestamp":1773321991051,"version":"3.50.1"},"reference-count":33,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5827","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2007,5,18]]},"abstract":"<jats:p>\n                    Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK\/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK\/PLK4 could induce amplification of centrioles in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.\n                  <\/jats:p>","DOI":"10.1126\/science.1142950","type":"journal-article","created":{"date-parts":[[2007,4,26]],"date-time":"2007-04-26T21:03:40Z","timestamp":1177621420000},"page":"1046-1050","source":"Crossref","is-referenced-by-count":223,"title":["Revisiting the Role of the Mother Centriole in Centriole Biogenesis"],"prefix":"10.1126","volume":"316","author":[{"given":"A.","family":"Rodrigues-Martins","sequence":"first","affiliation":[{"name":"Instituto Gulbenkian de Cie\u0302ncia, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal."},{"name":"Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK."},{"name":"Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy."}]},{"given":"M.","family":"Riparbelli","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Cie\u0302ncia, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal."},{"name":"Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK."},{"name":"Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy."}]},{"given":"G.","family":"Callaini","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Cie\u0302ncia, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal."},{"name":"Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK."},{"name":"Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy."}]},{"given":"D. M.","family":"Glover","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Cie\u0302ncia, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal."},{"name":"Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK."},{"name":"Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy."}]},{"given":"M.","family":"Bettencourt-Dias","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Cie\u0302ncia, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal."},{"name":"Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK."},{"name":"Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy."}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01128"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21117"},{"key":"e_1_3_1_4_2","unstructured":"J. Gall in Centrosomes in Development and Disease E. A. Nigg Ed. 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Foe, G. M. Odell, B. Edgar, in The Development of Drosophila melanogaster, M. Bate, A. Martinez Arias, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1993), pp. 149\u2013287.","journal-title":"The Development of Drosophila melanogaster"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.111.18.2697"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.45.2.306"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02179"},{"key":"e_1_3_1_25_2","unstructured":"Materials and methods are available as supporting online material on Science Online."},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200402130"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02028"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1.9.924"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90050-9"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.100.4.729"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00025-2"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1141314"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2006.01.005"},{"key":"e_1_3_1_34_2","unstructured":"We thank C. Ferreira and I. Ferreira for help with experiments; D. Johnston R. Basto and J. Raff for reagents; R. Martinho J. Leal M. Gatt R. Kuriyama and anonymous reviewers for comments on the manuscript; the Drosophila Instituto Gulbenkian de Ci\u00eancia (IGC) community G. Goshima and the M.B.-D. and D.M.G. groups for discussions; thebestgene.com for making transgenic flies; and the IGC imaging unit for help with image acquisition. We are grateful for grants from Cancer Research UK Funda\u00e7\u00e3o Calouste Gulbenkian and Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (POCI2010) and for an International Joint Project grant from the Royal Society for collaboration between the M.B.-D. and D.M.G. groups."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1142950","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T03:38:34Z","timestamp":1704857914000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1142950"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,18]]},"references-count":33,"journal-issue":{"issue":"5827","published-print":{"date-parts":[[2007,5,18]]}},"alternative-id":["10.1126\/science.1142950"],"URL":"https:\/\/doi.org\/10.1126\/science.1142950","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1086061.539027","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,18]]}}}