{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T02:07:16Z","timestamp":1775268436989,"version":"3.50.1"},"reference-count":28,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5894","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2008,9,5]]},"abstract":"<jats:p>During intracellular membrane trafficking and remodeling, protein complexes known as the ESCRTs (endosomal sorting complexes required for transport) interact with membranes and are required for budding processes directed away from the cytosol, including the budding of intralumenal vesicles to form multivesicular bodies; for the budding of some enveloped viruses; and for daughter cell scission in cytokinesis. We found that the ESCRT-III proteins CHMP2A and CHMP3 (charged multivesicular body proteins 2A and 3) could assemble in vitro into helical tubular structures that expose their membrane interaction sites on the outside of the tubule, whereas the AAA-type adenosine triphosphatase VPS4 could bind on the inside of the tubule and disassemble the tubes upon adenosine triphosphate hydrolysis. CHMP2A and CHMP3 copolymerized in solution, and their membrane targeting was cooperatively enhanced on planar lipid bilayers. Such helical CHMP structures could thus assemble within the neck of an inwardly budding vesicle, catalyzing late steps in budding under the control of VPS4.<\/jats:p>","DOI":"10.1126\/science.1161070","type":"journal-article","created":{"date-parts":[[2008,8,7]],"date-time":"2008-08-07T21:35:50Z","timestamp":1218144950000},"page":"1354-1357","source":"Crossref","is-referenced-by-count":323,"title":["Helical Structures of ESCRT-III Are Disassembled by VPS4"],"prefix":"10.1126","volume":"321","author":[{"given":"Suman","family":"Lata","sequence":"first","affiliation":[{"name":"Unit for Virus Host Cell Interaction, UMR 5233 UJF (Universite\u0301 Joseph Fourier)-EMBL (European Molecular Biology Laboratory)-CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France."},{"name":"Institut de Biologie Structurale UMR 5075 CEA (Comissariat a\u0300 l'E\u0301nergie Atomique)-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France."},{"name":"Institute of Biochemistry, Johann Wolfgang Goethe University, Max-von-Laue Stra\u00dfe 9, 60438 Frankfurt am Main, Germany."},{"name":"Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA."}]},{"given":"Guy","family":"Schoehn","sequence":"additional","affiliation":[{"name":"Unit for Virus Host Cell Interaction, UMR 5233 UJF (Universite\u0301 Joseph Fourier)-EMBL (European Molecular Biology Laboratory)-CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France."},{"name":"Institut de Biologie Structurale UMR 5075 CEA (Comissariat a\u0300 l'E\u0301nergie Atomique)-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France."},{"name":"Institute of Biochemistry, Johann Wolfgang Goethe University, Max-von-Laue Stra\u00dfe 9, 60438 Frankfurt am Main, Germany."},{"name":"Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA."}]},{"given":"Ankur","family":"Jain","sequence":"additional","affiliation":[{"name":"Unit for Virus Host Cell Interaction, UMR 5233 UJF (Universite\u0301 Joseph Fourier)-EMBL (European Molecular Biology Laboratory)-CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France."},{"name":"Institut de Biologie Structurale UMR 5075 CEA (Comissariat a\u0300 l'E\u0301nergie Atomique)-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France."},{"name":"Institute of Biochemistry, Johann Wolfgang Goethe University, Max-von-Laue Stra\u00dfe 9, 60438 Frankfurt am Main, Germany."},{"name":"Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA."}]},{"given":"Ricardo","family":"Pires","sequence":"additional","affiliation":[{"name":"Unit for Virus Host Cell Interaction, UMR 5233 UJF (Universite\u0301 Joseph Fourier)-EMBL (European Molecular Biology Laboratory)-CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France."},{"name":"Institut de Biologie Structurale UMR 5075 CEA (Comissariat a\u0300 l'E\u0301nergie Atomique)-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France."},{"name":"Institute of Biochemistry, Johann Wolfgang Goethe University, Max-von-Laue Stra\u00dfe 9, 60438 Frankfurt am Main, Germany."},{"name":"Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA."}]},{"given":"Jacob","family":"Piehler","sequence":"additional","affiliation":[{"name":"Unit for Virus Host Cell Interaction, UMR 5233 UJF (Universite\u0301 Joseph Fourier)-EMBL (European Molecular Biology Laboratory)-CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France."},{"name":"Institut de Biologie Structurale UMR 5075 CEA (Comissariat a\u0300 l'E\u0301nergie Atomique)-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France."},{"name":"Institute of Biochemistry, Johann Wolfgang Goethe University, Max-von-Laue Stra\u00dfe 9, 60438 Frankfurt am Main, Germany."},{"name":"Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA."}]},{"given":"Heinrich G.","family":"Go\u030bttlinger","sequence":"additional","affiliation":[{"name":"Unit for Virus Host Cell Interaction, UMR 5233 UJF (Universite\u0301 Joseph Fourier)-EMBL (European Molecular Biology Laboratory)-CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France."},{"name":"Institut de Biologie Structurale UMR 5075 CEA (Comissariat a\u0300 l'E\u0301nergie Atomique)-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France."},{"name":"Institute of Biochemistry, Johann Wolfgang Goethe University, Max-von-Laue Stra\u00dfe 9, 60438 Frankfurt am Main, Germany."},{"name":"Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, 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Siebert (Institut de Biologie Structurale) for advice on UROX. This work was supported by Deutsche Forschungsgemeinschaft (SPP 1175) (W.W.) the Agence Nationale de la Recherche sur le SIDA (W.W.) Universit\u00e9 Joseph Fourier (W.W.) the Agence Nationale de la Recherche (G.S.) the CNRS (G.S.) the NIH (grant AI29873 H.G.) and by postdoctoral fellowships from the European Molecular Biology Organization (S.L.) and the International Human Frontier Science Program Organization (S.L.). The EM map has been deposited at the European Bioinformatics Institute accession code EMD-1536."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1161070","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T03:23:28Z","timestamp":1704857008000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1161070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9,5]]},"references-count":28,"journal-issue":{"issue":"5894","published-print":{"date-parts":[[2008,9,5]]}},"alternative-id":["10.1126\/science.1161070"],"URL":"https:\/\/doi.org\/10.1126\/science.1161070","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1124092.581218","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,9,5]]}}}