{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:53:45Z","timestamp":1793199225820,"version":"4.5.0"},"reference-count":45,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6121","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2013,2,15]]},"abstract":"<jats:title>Genome Editing<\/jats:title>\n                  <jats:p>\n                    Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound to a CRISPR-associated endonuclease (see the Perspective by\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6121\" page=\"768\" related-article-type=\"in-this-issue\" vol=\"339\" xlink:href=\"10.1126\/science.1234726\">\n                      <jats:bold>van der Oost<\/jats:bold>\n                    <\/jats:related-article>\n                    ).\n                    <jats:bold>\n                      Cong\n                      <jats:italic>et al.<\/jats:italic>\n                    <\/jats:bold>\n                    (p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6121\" page=\"819\" related-article-type=\"in-this-issue\" vol=\"339\" xlink:href=\"10.1126\/science.1231143\">819<\/jats:related-article>\n                    , published online 3 January) and\n                    <jats:bold>\n                      Mali\n                      <jats:italic>et al.<\/jats:italic>\n                    <\/jats:bold>\n                    (p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6121\" page=\"823\" related-article-type=\"in-this-issue\" vol=\"339\" xlink:href=\"10.1126\/science.1232033\">823<\/jats:related-article>\n                    , published online 3 January) adapted this defense system to function as a genome editing tool in eukaryotic cells.\n                  <\/jats:p>","DOI":"10.1126\/science.1232033","type":"journal-article","created":{"date-parts":[[2013,1,4]],"date-time":"2013-01-04T00:46:42Z","timestamp":1357260402000},"page":"823-826","source":"Crossref","is-referenced-by-count":8761,"title":["RNA-Guided Human Genome Engineering via Cas9"],"prefix":"10.1126","volume":"339","author":[{"given":"Prashant","family":"Mali","sequence":"first","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luhan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, 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