{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T15:08:29Z","timestamp":1793200109935,"version":"4.5.0"},"reference-count":58,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6440","funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["JI 289\/1-1"],"award-info":[{"award-number":["JI 289\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Joseph J. Jacobs Institute for Molecular Engineering for Medicine"},{"DOI":"10.13039\/100000152","name":"NSF Division of Molecular and Cellular Biosciences","doi-asserted-by":"crossref","award":["MCB-1513007"],"award-info":[{"award-number":["MCB-1513007"]}],"id":[{"id":"10.13039\/100000152","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2019,5,10]]},"abstract":"<jats:title>The right bond in the right place<\/jats:title>\n                  <jats:p>\n                    Enzymes excel at specificity because of their constrained active sites. With appropriate evolutionary pressure, they can be made to differentiate between similar substrates or between positions on a single substrate. Cho\n                    <jats:italic>et al.<\/jats:italic>\n                    used directed evolution to generate cytochrome P450 variants that target different C\u2013H bonds in substrates, forming lactam rings of varying size (see the Perspective by Hepworth and Flitsch). The enzyme directs amidation to the desired position and simultaneously prevents other side reactions.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Science<\/jats:italic>\n                    , this issue p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6440\" page=\"575\" related-article-type=\"in-this-issue\" vol=\"364\" xlink:href=\"10.1126\/science.aaw9068\">575<\/jats:related-article>\n                    ; see also p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6440\" page=\"529\" related-article-type=\"in-this-issue\" vol=\"364\" xlink:href=\"10.1126\/science.aax3335\">529<\/jats:related-article>\n                  <\/jats:p>","DOI":"10.1126\/science.aaw9068","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T19:05:25Z","timestamp":1557428725000},"page":"575-578","source":"Crossref","is-referenced-by-count":59,"title":["Site-selective enzymatic C\u2012H amidation for synthesis of diverse lactams"],"prefix":"10.1126","volume":"364","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7564-5378","authenticated-orcid":true,"given":"Inha","family":"Cho","sequence":"first","affiliation":[{"name":"Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5143-4875","authenticated-orcid":true,"given":"Zhi-Jun","family":"Jia","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4027-364X","authenticated-orcid":true,"given":"Frances H.","family":"Arnold","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1114731"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0cs00182a"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201201666"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.5b02818"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1607"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar200185g"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4CS00137K"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr900239n"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10785"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14255"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19811"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201006368"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-018-0087-7"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8OB02272K"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7CS00064B"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201600489"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1085515"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr9002193"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00373"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/cs300001x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2285"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7CC00368D"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2017.06.005"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2018.10.004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0808-5"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja509308v"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2783"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200400657"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aap7503"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b02769"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/cs5018612"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201812968"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201507630"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1097\/MRM.0b013e3283587727"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(01)80290-2"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8OB01833B"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr4005549"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar4239"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/sb300037w"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1318"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(76)44034-X"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90643-9"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.1594"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aah6219"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature24996"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.7b00548"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.6b02550"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1278"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.5b00056"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b11455"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1380553"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1039\/c39800000736"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5MD00340G"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/0040-4039(90)80147-E"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja507576a"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo00182a022"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1080\/00304949309457960"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2007.11.016"}],"updated-by":[{"DOI":"10.1126\/science.aba6100","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2020,1,2]],"date-time":"2020-01-02T00:00:00Z","timestamp":1577923200000},"record-id":"21884"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1126\/science.aaw9068","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.aaw9068","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T18:54:06Z","timestamp":1705344846000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.aaw9068"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,10]]},"references-count":58,"journal-issue":{"issue":"6440","published-print":{"date-parts":[[2019,5,10]]}},"alternative-id":["10.1126\/science.aaw9068"],"URL":"https:\/\/doi.org\/10.1126\/science.aaw9068","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.735752099.793560980","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.735752099.793560238","asserted-by":"object"}],"has-preprint":[{"id-type":"doi","id":"10.26434\/chemrxiv.7711418.v1","asserted-by":"object"},{"id-type":"doi","id":"10.26434\/chemrxiv.7711418.v2","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,10]]}}}