{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T16:21:08Z","timestamp":1756311668954,"version":"3.41.2"},"reference-count":62,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"771","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Signal."],"published-print":{"date-parts":[[2023,2,7]]},"abstract":"<jats:p>\n            Stormorken syndrome is a multiorgan hereditary disease caused by dysfunction of the endoplasmic reticulum (ER) Ca\n            <jats:sup>2+<\/jats:sup>\n            sensor protein STIM1, which forms the Ca\n            <jats:sup>2+<\/jats:sup>\n            release\u2013activated Ca\n            <jats:sup>2+<\/jats:sup>\n            (CRAC) channel together with the plasma membrane channel Orai1. ER Ca\n            <jats:sup>2+<\/jats:sup>\n            store depletion activates STIM1 by releasing the intramolecular \u201cclamp\u201d formed between the coiled coil 1 (CC1) and CC3 domains of the protein, enabling the C terminus to extend and interact with Orai1. The most frequently occurring mutation in patients with Stormorken syndrome is R304W, which destabilizes and extends the STIM1 C terminus independently of ER Ca\n            <jats:sup>2+<\/jats:sup>\n            store depletion, causing constitutive binding to Orai1 and CRAC channel activation. We found that in cis deletion of one amino acid residue, Glu\n            <jats:sup>296<\/jats:sup>\n            (which we called E296del) reversed the pathological effects of R304W. Homozygous Stim1 E296del+R304W mice were viable and phenotypically indistinguishable from wild-type mice. NMR spectroscopy, molecular dynamics simulations, and cellular experiments revealed that although the R304W mutation prevented CC1 from interacting with CC3, the additional deletion of Glu\n            <jats:sup>296<\/jats:sup>\n            opposed this effect by enabling CC1-CC3 binding and restoring the CC domain interactions within STIM1 that are critical for proper CRAC channel function. Our results provide insight into the activation mechanism of STIM1 by clarifying the molecular basis of mutation-elicited protein dysfunction and pathophysiology.\n          <\/jats:p>","DOI":"10.1126\/scisignal.add0509","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T18:58:07Z","timestamp":1675796287000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":3,"title":["A single amino acid deletion in the ER Ca\n            <sup>2+<\/sup>\n            sensor STIM1 reverses the in vitro and in vivo effects of the Stormorken syndrome\u2013causing R304W mutation"],"prefix":"10.1126","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1727-2451","authenticated-orcid":true,"given":"Thilini H.","family":"Gamage","sequence":"first","affiliation":[{"name":"Department of Medical Genetics, Oslo University Hospital and University of Oslo, 0450 Oslo, 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Medical Genetics, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway."}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0511-6112","authenticated-orcid":true,"given":"William Edward","family":"Louch","sequence":"additional","affiliation":[{"name":"Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway."}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7900-1027","authenticated-orcid":true,"given":"Gjermund","family":"Gunnes","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 1430 \u00c5s, Norway."}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5117-4604","authenticated-orcid":true,"given":"Helen","family":"Pullisaar","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0455 Oslo, Norway."}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5743-4297","authenticated-orcid":true,"given":"Janne Elin","family":"Reseland","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0455 Oslo, Norway."}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6361-7644","authenticated-orcid":true,"given":"Staale Petter","family":"Lyngstadaas","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0455 Oslo, Norway."}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1434-0957","authenticated-orcid":true,"given":"Asbj\u00f8rn","family":"Holmgren","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway."}]},{"given":"Silja S.","family":"Amundsen","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway."}]},{"given":"Petr","family":"Rathner","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry and Institute of Inorganic Chemistry, Johannes Kepler University Linz, Altenbergerstrasse 69, 4040 Linz, Austria."},{"name":"Institut f\u00fcr Analytische Chemie, University of Vienna, W\u00e4hringer Stra\u00dfe 38, 1090 Wien, Austria."}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0795-9594","authenticated-orcid":true,"given":"Linda","family":"Cerofolini","sequence":"additional","affiliation":[{"name":"Magnetic Resonance Center, University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine, 50019 Sesto Fiorentino, Italy."}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7708-9208","authenticated-orcid":true,"given":"Enrico","family":"Ravera","sequence":"additional","affiliation":[{"name":"Magnetic Resonance Center, University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine, 50019 Sesto Fiorentino, 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