{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T19:30:24Z","timestamp":1779910224969,"version":"3.53.1"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            is well known for its metabolic capabilities, but recently, it has been shown to exhibit resistance to a wide range of antibiotics. In\n            <jats:italic>P. putida<\/jats:italic>\n            DOT-T1E, the TtgABC efflux pump, which has a broad substrate specificity, extrudes antibiotics such as ampicillin, carbenicillin, tetracycline, nalidixic acid, and chloramphenicol. We have analyzed the expression of the\n            <jats:italic>ttgABC<\/jats:italic>\n            efflux pump operon and its regulatory gene,\n            <jats:italic>ttgR<\/jats:italic>\n            , in response to several structurally unrelated antibiotics at the transcriptional level and investigated the role of the TtgR protein in this process.\n            <jats:italic>ttgABC<\/jats:italic>\n            and\n            <jats:italic>ttgR<\/jats:italic>\n            are expressed in vivo at a moderate basal level, which increases in the presence of hydrophobic antibiotics like chloramphenicol and tetracycline. In vitro experiments show that, in the absence of inducers, TtgR binds to a palindromic operator site which overlaps both\n            <jats:italic>ttgABC<\/jats:italic>\n            and\n            <jats:italic>ttgR<\/jats:italic>\n            promoters and dissociates from it in the presence of chloramphenicol and tetracycline. These results suggest that the TtgR repressor is able to bind to structurally different antibiotics, which allows induction of TtgABC multidrug efflux pump expression in response to these antimicrobial agents. This is the first case in which the expression of a drug transporter of the resistance-nodulation-division family has been shown to be regulated directly by antibiotics.\n          <\/jats:p>","DOI":"10.1128\/aac.47.10.3067-3072.2003","type":"journal-article","created":{"date-parts":[[2003,9,23]],"date-time":"2003-09-23T22:25:36Z","timestamp":1064355936000},"page":"3067-3072","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":135,"title":["Antibiotic-Dependent Induction of\n            <i>Pseudomonas putida<\/i>\n            DOT-T1E TtgABC Efflux Pump Is Mediated by the Drug Binding Repressor TtgR"],"prefix":"10.1128","volume":"47","author":[{"given":"Wilson","family":"Tera\u0301n","sequence":"first","affiliation":[{"name":"Consejo Superior de Investigaciones Cienti\u0301ficas, Estacio\u0301n Experimental del Zaidi\u0301n, Department of Plant Biochemistry, Molecular and Cellular Biology, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Felipe","sequence":"additional","affiliation":[{"name":"Consejo Superior de Investigaciones Cienti\u0301ficas, Estacio\u0301n Experimental del Zaidi\u0301n, Department of Plant Biochemistry, Molecular and Cellular Biology, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Segura","sequence":"additional","affiliation":[{"name":"Consejo Superior de Investigaciones Cienti\u0301ficas, Estacio\u0301n Experimental del Zaidi\u0301n, Department of Plant Biochemistry, Molecular and Cellular Biology, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Rojas","sequence":"additional","affiliation":[{"name":"Consejo Superior de Investigaciones Cienti\u0301ficas, Estacio\u0301n Experimental del Zaidi\u0301n, Department of Plant Biochemistry, Molecular and Cellular Biology, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan-Luis","family":"Ramos","sequence":"additional","affiliation":[{"name":"Consejo Superior de Investigaciones Cienti\u0301ficas, Estacio\u0301n Experimental del Zaidi\u0301n, Department of Plant Biochemistry, Molecular and Cellular Biology, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a-Trinidad","family":"Gallegos","sequence":"additional","affiliation":[{"name":"Consejo Superior de Investigaciones Cienti\u0301ficas, Estacio\u0301n Experimental del Zaidi\u0301n, Department of Plant Biochemistry, Molecular and Cellular Biology, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.10.2067"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.4.938-944.1998"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/8.12.1259"},{"key":"e_1_3_2_5_2","unstructured":"Ausubel F. 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