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The diversity of autochthonous<jats:italic>Pseudomonas<\/jats:italic>populations present at two beaches (Carnota municipality) was analyzed using culture-independent and culture-dependent methods. The first analysis involved the screening of an<jats:italic>rpoD<\/jats:italic>gene library. The second involved the isolation of 94<jats:italic>Pseudomonas<\/jats:italic>strains that were able to grow on selective media by direct plating or after serial enrichments on several carbon sources: biphenyl, gentisate, hexadecane, methylnaphthalene, naphthalene, phenanthrene, salicylate, xylene, and succinate. Eight denitrifying<jats:italic>Pseudomonas<\/jats:italic>strains were also isolated by their ability to grow anaerobically with nitrate. The calculated coverage index for<jats:italic>Pseudomonas<\/jats:italic>species was 89% when clones and isolates were considered together, and there were 29 phylospecies detected. The most abundant were members of the species<jats:italic>P. stutzeri<\/jats:italic>,<jats:italic>P<\/jats:italic>.<jats:italic>putida<\/jats:italic>,<jats:italic>P. anguilliseptica<\/jats:italic>, and<jats:italic>P. oleovorans<\/jats:italic>. Thirty-one isolates could not be identified at the species level and were considered representatives of 16 putative novel<jats:italic>Pseudomonas<\/jats:italic>species. One isolate was considered representative of a novel<jats:italic>P. stutzeri<\/jats:italic>genomovar. Concordant results were obtained when the diversities of the cloned DNA library and the cultured strains were compared. The clone library obtained by the<jats:italic>rpoD<\/jats:italic>PCR method was a useful tool for evaluating<jats:italic>Pseudomonas<\/jats:italic>communities and also for microdiversity studies of<jats:italic>Pseudomonas<\/jats:italic>populations.<\/jats:p>","DOI":"10.1128\/aem.01741-10","type":"journal-article","created":{"date-parts":[[2010,12,4]],"date-time":"2010-12-04T05:49:03Z","timestamp":1291441743000},"page":"1076-1085","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["<i>Pseudomonas<\/i>Diversity in Crude-Oil-Contaminated Intertidal Sand Samples Obtained after the<i>Prestige<\/i>Oil Spill"],"prefix":"10.1128","volume":"77","author":[{"given":"Magdalena","family":"Mulet","sequence":"first","affiliation":[{"name":"Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanc\u0327ats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoyla","family":"David","sequence":"additional","affiliation":[{"name":"Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanc\u0327ats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balbina","family":"Nogales","sequence":"additional","affiliation":[{"name":"Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanc\u0327ats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Bosch","sequence":"additional","affiliation":[{"name":"Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanc\u0327ats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Lalucat","sequence":"additional","affiliation":[{"name":"Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanc\u0327ats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Garci\u0301a-Valde\u0301s","sequence":"additional","affiliation":[{"name":"Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanc\u0327ats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2008.04.003"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01776-08"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_3_5_2","first-page":"865","year":"1981","unstructured":"Aragno, M., and H. 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