{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T19:20:20Z","timestamp":1775416820484,"version":"3.50.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2008,4,15]],"date-time":"2008-04-15T00:00:00Z","timestamp":1208217600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            According to in silico analysis, the genome of\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            KT2440 encodes at least four Zn\/Cd\/Pb efflux transporters\u2014two P-type ATPases (CadA1 and CadA2) and two\n            <jats:italic>czc<\/jats:italic>\n            chemiosmotic transporters (CzcCBA1 and CzcCBA2). In this study we showed that all these transporters are functional, but under laboratory conditions only two of them were involved in the mediation of heavy metal resistance in\n            <jats:italic>P. putida<\/jats:italic>\n            KT2440. CadA2 conferred Cd\n            <jats:sup>2+<\/jats:sup>\n            and Pb\n            <jats:sup>2+<\/jats:sup>\n            resistance, whereas CzcCBA1 was involved in export of Zn\n            <jats:sup>2+<\/jats:sup>\n            , Cd\n            <jats:sup>2+<\/jats:sup>\n            , and possibly Pb\n            <jats:sup>2+<\/jats:sup>\n            . CadA1, although nonfunctional in\n            <jats:italic>P. putida<\/jats:italic>\n            , improved Zn\n            <jats:sup>2+<\/jats:sup>\n            resistance and slightly improved Cd\n            <jats:sup>2+<\/jats:sup>\n            resistance when it was expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . CzcCBA2 contributed to Zn resistance of a\n            <jats:italic>czcA1<\/jats:italic>\n            -defective\n            <jats:italic>P. putida<\/jats:italic>\n            strain or when the CzcA2 subunit was overexpressed in a transporter-deficient strain. It seemed that CzcA2 could complex with CzcC1 and CzcB1 subunits and therefore complement the loss of CzcA1. The CzcCBA2 transporter itself, however, did not function. Expression of\n            <jats:italic>cadA1<\/jats:italic>\n            ,\n            <jats:italic>cadA2<\/jats:italic>\n            , and\n            <jats:italic>czcCBA1<\/jats:italic>\n            was induced by heavy metals, and the expression levels were dependent on the growth medium and growth phase. Expression of\n            <jats:italic>cadA2<\/jats:italic>\n            and\n            <jats:italic>czcCBA1<\/jats:italic>\n            was nonspecific; both genes were induced by Zn\n            <jats:sup>2+<\/jats:sup>\n            , Cd\n            <jats:sup>2+<\/jats:sup>\n            , Pb\n            <jats:sup>2+<\/jats:sup>\n            , Ni\n            <jats:sup>2+<\/jats:sup>\n            , Co\n            <jats:sup>2+<\/jats:sup>\n            , and Hg\n            <jats:sup>2+<\/jats:sup>\n            . On the other hand, remarkably, expression of\n            <jats:italic>cadA1<\/jats:italic>\n            was induced only by Zn\n            <jats:sup>2+<\/jats:sup>\n            . Possible roles of distinct but simultaneously functioning transporters are discussed.\n          <\/jats:p>","DOI":"10.1128\/jb.01494-07","type":"journal-article","created":{"date-parts":[[2007,12,8]],"date-time":"2007-12-08T01:53:35Z","timestamp":1197078815000},"page":"2680-2689","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":101,"title":["Interplay of Different Transporters in the Mediation of Divalent Heavy Metal Resistance in\n            <i>Pseudomonas putida<\/i>\n            KT2440"],"prefix":"10.1128","volume":"190","author":[{"given":"Anu","family":"Leedja\u0308rv","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, FIN-00014 Helsinki, Finland"},{"name":"Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Ivask","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marko","family":"Virta","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, FIN-00014 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.22.6876-6881.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0378-1119(81)90080-9","volume":"16","year":"1981","unstructured":"Bagdasarian, M., R. Lurz, B. Ruckert, F. C. Franklin, M. M. Bagdasarian, J. Frey, and K. N. Timmis. 1981. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene 16 : 237-247.","journal-title":"Gene"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0014-5793(00)01509-X","volume":"473","year":"2000","unstructured":"Binet, M. R., and R. K. Poole. 2000. Cd(II), Pb(II) and Zn(II) ions regulate expression of the metal-transporting P-type ATPase ZntA in Escherichia coli. FEBS Lett. 473 : 67-70.","journal-title":"FEBS Lett."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1046\/j.1365-2958.1999.01229.x","volume":"31","year":"1999","unstructured":"Brocklehurst, K. R., J. L. Hobman, B. Lawley, L. Blank, S. J. Marshall, N. L. Brown, and A. P. Morby. 1999. ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli. Mol. Microbiol. 31 : 893-902.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00095-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1111\/j.1462-2920.2003.00463.x","volume":"5","year":"2003","unstructured":"Canovas, D., I. Cases., and V. de Lorenzo. 2003. Heavy metal tolerance and metal homeostasis in Pseudomonas putida as revealed by complete genome analysis. Environ. Microbiol. 5 : 1242-1256.","journal-title":"Environ. Microbiol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/0022-2836(80)90283-1","volume":"138","year":"1980","unstructured":"Casadaban, M. J., and S. N. Cohen. 1980. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138 : 179-207.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.06.001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.13.3804-3812.2003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.15.4664-4667.2001"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.8.2385-2393.1999"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0378-1119(99)00349-2","volume":"238","year":"1999","unstructured":"Hassan, M. T., D. van der Lelie, D. Springael, U. Romling, N. Ahmed, and M. Mergeay. 1999. Identification of a gene cluster, czr, involved in cadmium and zinc resistance in Pseudomonas aeruginosa. Gene 238 : 417-425.","journal-title":"Gene"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-1119(98)00130-9","volume":"212","year":"1998","unstructured":"Hoang, T. T., R. R. Karkhoff-Schweizer, A. J. Kutchma, and H. P. Schweizer. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212 : 77-86.","journal-title":"Gene"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1574-6968.2006.00505.x","volume":"267","year":"2007","unstructured":"Hu, N., and B. Zhao. 2007. Key genes involved in heavy-metal resistance in Pseudomonas putida CD2. FEMS Microbiol. Lett. 267 : 17-22.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2004.10.002"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1007\/BF01569936","volume":"14","year":"1995","unstructured":"LaRossa, R. A., D. R. Smulski, and T. K. Van Dyk. 1995. Interaction of lead nitrate and cadmium chloride with Escherichia coli K-12 and Salmonella typhimurium global regulatory mutants. J. Ind. Microbiol. 14 : 252-258.","journal-title":"J. Ind. Microbiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.4.1437-1444.2001"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"1910","DOI":"10.1016\/j.chemosphere.2006.01.026","volume":"64","year":"2006","unstructured":"Leedj\u00e4rv, A., A. Ivask, M. Virta, and A. Kahru. 2006. Analysis of bioavailable phenols from natural samples by recombinant luminescent bacterial sensors. Chemosphere 64 : 1910-1919.","journal-title":"Chemosphere"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.15.4354-4361.2003"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.20.5864-5871.2000"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0168-6445(03)00048-2","volume":"27","year":"2003","unstructured":"Nies, D. H. 2003. Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiol. Rev. 27 : 313-339.","journal-title":"FEMS Microbiol. Rev."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.2.896-900.1989"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"7351","DOI":"10.1073\/pnas.86.19.7351","volume":"86","year":"1989","unstructured":"Nies, D. H., A. Nies, L. Chu, and S. Silver. 1989. Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus. Proc. Natl. Acad. Sci. USA 86 : 7351-7355.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"3544","DOI":"10.1073\/pnas.86.10.3544","volume":"86","year":"1989","unstructured":"Nucifora, G., L. Chu, T. K. Misra, and S. Silver. 1989. Cadmium resistance from Staphylococcus aureus plasmid pI258 cadA gene results from a cadmium-efflux ATPase. Proc. Natl. Acad. Sci. USA 86 : 3544-3548.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.6.2911-2919.1990"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"8761","DOI":"10.1074\/jbc.M312080200","volume":"279","year":"2004","unstructured":"Perron, K., O. Caille, C. Rossier, C. Van Delden, J. L. Dumas, and T. Kohler. 2004. CzcR-CzcS, a two-component system involved in heavy metal and carbapenem resistance in Pseudomonas aeruginosa. J. Biol. Chem. 279 : 8761-8768.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1073\/pnas.87.10.3846","volume":"87","year":"1990","unstructured":"Ralston, D. M., and T. V. O'Halloran. 1990. Ultrasensitivity and heavy-metal selectivity of the allosterically modulated MerR transcription complex. Proc. Natl. Acad. Sci. USA 87 : 3846-3850.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"14326","DOI":"10.1073\/pnas.94.26.14326","volume":"94","year":"1997","unstructured":"Rensing, C., B. Mitra, and B. P. Rosen. 1997. The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase. Proc. Natl. Acad. Sci. USA 94 : 14326-14331.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1111\/j.1574-6968.2000.tb09320.x","volume":"191","year":"2000","unstructured":"Rossbach, S., T. L. Wilson, M. L. Kukuk, and H. A. Carty. 2000. Elevated zinc induces siderophore biosynthesis genes and a zntA-like gene in Pseudomonas fluorescens. FEMS Microbiol. Lett. 191 : 61-70.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_32_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"3873","DOI":"10.1074\/jbc.275.6.3873","volume":"275","year":"2000","unstructured":"Sharma, R., C. Rensing, B. P. Rosen, and B. Mitra. 2000. The ATP hydrolytic activity of purified ZntA, a Pb(II)\/Cd(II)\/Zn(II)-translocating ATPase from Escherichia coli. J. Biol. Chem. 275 : 3873-3878.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1007\/s10295-005-0019-6","volume":"32","year":"2005","unstructured":"Silver, S., and L. T. Phung. 2005. A bacterial view of the periodic table: genes and proteins for toxic inorganic ions. J. Ind. Microbiol. Biotechnol. 32 : 587-605.","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/35023079"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1046\/j.1462-2920.2002.00365.x","volume":"4","year":"2002","unstructured":"Timmis, K. N. 2002. Pseudomonas putida: a cosmopolitan opportunist par excellence. Environ. Microbiol. 4 : 779-781.","journal-title":"Environ. Microbiol."},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1046\/j.1365-2958.1997.d01-1866.x","volume":"23","year":"1997","unstructured":"van der Lelie, D., T. Schwuchow, U. Schwidetzky, S. Wuertz, W. Baeyens, M. Mergeay, and D. H. Nies. 1997. Two-component regulatory system involved in transcriptional control of heavy-metal homoeostasis in Alcaligenes eutrophus. Mol. Microbiol. 23 : 493-503.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.16.4024-4029.1998"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.23.7643-7649.1991"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01494-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01494-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:45:15Z","timestamp":1627580715000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.01494-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4,15]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2008,4,15]]}},"alternative-id":["10.1128\/JB.01494-07"],"URL":"https:\/\/doi.org\/10.1128\/jb.01494-07","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4,15]]},"assertion":[{"value":"2007-09-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-11-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}