{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:42:32Z","timestamp":1791171752306,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Analysis of IS\n CR8<\/jats:italic>\n (IS\n Pps1<\/jats:italic>\n ) revealed that this group of insertion elements has to be subdivided into three subgroups: IS\n CR8<\/jats:italic>\n , IS\n CR22<\/jats:italic>\n , and IS\n CR23<\/jats:italic>\n . The distinction of three subgroups is supported by phylogenetic analysis of the transposase open reading frames (ORFs). Comparison of over 20 complete and partial IS\n CR8<\/jats:italic>\n \/\n 22\/23<\/jats:italic>\n elements identified\n ori<\/jats:italic>\n IS candidate sequences for all groups and a\n ter<\/jats:italic>\n IS candidate sequence for IS\n CR8<\/jats:italic>\n . The\n ori<\/jats:italic>\n IS sequences, their distance to the transposase ORFs, and the sequence of this intervening region are group specific, further supporting the definition of two new IS\n CR<\/jats:italic>\n elements. IS\n CR8\/22\/23<\/jats:italic>\n have a very broad host range, including Gram-positive and Gram-negative bacteria, among which are several (opportunistic) pathogens. The IS often resides on plasmids or in the vicinity of other mobile elements and is mostly associated with genes for the degradation of halo- or nitro-aromatics. However, in one case IS\n CR8<\/jats:italic>\n was found in the neighborhood of an antibiotic resistance determinant in\n Klebsiella pneumoniae<\/jats:italic>\n . IS\n CR8<\/jats:italic>\n resembles other IS91 family elements in mediating genetic rearrangements by homologous recombination between two copies. In\n Delftia acidovorans<\/jats:italic>\n this led to the loss of the genes encoding dichlorprop cleavage. In conclusion, this study shows that IS\n CR8<\/jats:italic>\n could be a fully functional and active member of the IS\n 91<\/jats:italic>\n family of insertion elements.\n <\/jats:p>","DOI":"10.1128\/aac.00006-10","type":"journal-article","created":{"date-parts":[[2010,7,13]],"date-time":"2010-07-13T03:59:24Z","timestamp":1278993564000},"page":"4321-4328","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Structural Characterization of IS\n CR8<\/i>\n , IS\n CR22<\/i>\n , and IS\n CR23<\/i>\n , Subgroups of IS\n 91<\/i>\n -Like Insertion Elements"],"prefix":"10.1128","volume":"54","author":[{"given":"Kathleen M.","family":"Schleinitz","sequence":"first","affiliation":[{"name":"UFZ Centre for Environmental Research Leipzig-Halle, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Vallaeys","sequence":"additional","affiliation":[{"name":"Laboratoire ECOLAG, UMR CNRS-UMII 5119, Universite\u0301 Montpellier II, cc 093, Place E. Bataillon, F-34095 Montpellier Cedex 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Kleinsteuber","sequence":"additional","affiliation":[{"name":"UFZ Centre for Environmental Research Leipzig-Halle, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01432.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S146625230800159X"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19970315)13:3<233::AID-YEA88>3.0.CO;2-E"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.22.10881"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02261.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2002.tb01020.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn166"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01819.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1244"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00616-07"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.5.1922"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00247.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.11.2200"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.9.5357-5365.2004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj014"},{"key":"e_1_3_2_18_2","first-page":"2","volume":"3","year":"1999","unstructured":"S\u00f8rensen, A. 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