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We sequenced, assembled, and annotated the Bp1651 genome and analyzed the sequence using comparative genomic analyses with susceptible strains, keyword searches of the annotation, publicly available antimicrobial resistance prediction tools, and published reports. More than 100 genes in the Bp1651 sequence were identified as potentially contributing to antimicrobial resistance. Most notably, we identified three previously uncharacterized point mutations in\n                    <jats:italic>penA<\/jats:italic>\n                    , which codes for a class A \u03b2-lactamase and was previously implicated in resistance to \u03b2-lactam antibiotics. The mutations result in amino acid changes T147A, D240G, and V261I. When individually introduced into select agent-excluded\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    strain Bp82, D240G was found to contribute to ceftazidime resistance and T147A contributed to amoxicillin-clavulanic acid and imipenem resistance. This study provides the first evidence that mutations in\n                    <jats:italic>penA<\/jats:italic>\n                    may alter susceptibility to carbapenems in\n                    <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n                    . Another mutation of interest was a point mutation affecting the dihydrofolate reductase gene\n                    <jats:italic>folA<\/jats:italic>\n                    , which likely explains the trimethoprim resistance of this strain. Bp1651 was susceptible to aminoglycosides likely because of a frameshift in the\n                    <jats:italic>amrB<\/jats:italic>\n                    gene, the transporter subunit of the AmrAB-OprA efflux pump. These findings expand the role of\n                    <jats:italic>penA<\/jats:italic>\n                    to include resistance to carbapenems and may assist in the development of molecular diagnostics that predict antimicrobial resistance and provide guidance for treatment of melioidosis.\n                  <\/jats:p>","DOI":"10.1128\/aac.00010-17","type":"journal-article","created":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T20:30:32Z","timestamp":1491856232000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Antibiotic Resistance Markers in Burkholderia pseudomallei Strain Bp1651 Identified by Genome Sequence Analysis"],"prefix":"10.1128","volume":"61","author":[{"given":"Julia V.","family":"Bugrysheva","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sue","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay E.","family":"Gee","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mindy G.","family":"Elrod","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex R.","family":"Hoffmaster","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linnell B.","family":"Randall","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunisa","family":"Chirakul","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apichai","family":"Tuanyok","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert P.","family":"Schweizer","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M.","family":"Weigel","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1204699"},{"key":"e_1_3_3_3_2","first-page":"1","article-title":"Melioidosis cases and selected reports of occupational exposures to Burkholderia pseudomallei\u2014United States, 2008-2013","volume":"64","author":"Benoit TJ","year":"2015","unstructured":"Benoit TJ BlaneyDD GeeJE ElrodMG HoffmasterAR DokerTJ BowerWA WalkeHT. 2015. 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