{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:23:43Z","timestamp":1791307423802,"version":"4.3.1"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Five different strains of bacteria belonging to the family\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            were isolated from two patients hospitalized in the intensive care unit of the Central Military Hospital of Algiers, Algeria. All five strains, one\n            <jats:italic>Providencia stuartii<\/jats:italic>\n            strain, two\n            <jats:italic>Escherichia coli<\/jats:italic>\n            strains, and two\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            strains, were intermediate or resistant to all \u03b2-lactams, including carbapenems. Synergy between imipenem and EDTA was observed for all five strains. The results of the PCR experiment confirmed the presence of a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>VIM<\/jats:sub>\n            gene in all five strains. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>VIM<\/jats:sub>\n            genes were located as part of a class 1 integron on a 180-kb conjugative plasmid. They encoded a novel metallo-\u03b2-lactamase designated VIM-19, which differed from the parental enzyme VIM-1 by only two substitutions: Ser228Arg, previously observed in the closely related enzyme VIM-4, and Asn215Lys, not previously observed in other VIM-type carbapenemases. VIM-19 was further characterized after purification through determination of its kinetic constants. This enzyme was inhibited by EDTA and hydrolyzed penicillins, cephalosporins, and carbapenems, as observed for other VIM-type carbapenemases but with greater catalytic efficiency against penicillins than VIM-1. VIM-19 is the first carbapenemase enzyme identified from an isolate from Algeria. These results confirm the emergence of VIM-4-like enzymes in members of the family\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            from Mediterranean countries.\n          <\/jats:p>","DOI":"10.1128\/aac.00017-09","type":"journal-article","created":{"date-parts":[[2009,11,10]],"date-time":"2009-11-10T03:07:02Z","timestamp":1257822422000},"page":"466-470","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Novel VIM Metallo-\u03b2-Lactamase Variant from Clinical Isolates of\n            <i>Enterobacteriaceae<\/i>\n            from Algeria"],"prefix":"10.1128","volume":"54","author":[{"given":"Fre\u0301de\u0301ric","family":"Robin","sequence":"first","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bacte\u0301riologie Clinique, Centre de Biologie, Clermont-Ferrand F-63003, France"},{"name":"Universite\u0301 d'Auvergne, UFR Me\u0301decine, Laboratoire de Bacte\u0301riologie, JE2526, USC INRA 2018, Clermont-Ferrand F-63001, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadjet","family":"Aggoune-Khinache","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Central de l'Arme\u0301e, Laboratoire de Microbiologie, Algiers, Algeria"},{"name":"Universite\u0301 d'Alger, Faculte\u0301 de Me\u0301decine, Laboratoire de Microbiologie, Algiers, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Delmas","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bacte\u0301riologie Clinique, Centre de Biologie, Clermont-Ferrand F-63003, France"},{"name":"Universite\u0301 d'Auvergne, UFR Me\u0301decine, Laboratoire de Bacte\u0301riologie, JE2526, USC INRA 2018, Clermont-Ferrand F-63001, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malek","family":"Naim","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Central de l'Arme\u0301e, Laboratoire de Microbiologie, Algiers, Algeria"},{"name":"Universite\u0301 d'Alger, Faculte\u0301 de Me\u0301decine, Laboratoire de Microbiologie, Algiers, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Bonnet","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bacte\u0301riologie Clinique, Centre de Biologie, Clermont-Ferrand F-63003, France"},{"name":"Universite\u0301 d'Auvergne, UFR Me\u0301decine, Laboratoire de Bacte\u0301riologie, JE2526, USC INRA 2018, Clermont-Ferrand F-63001, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3061-3068.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1936-1942.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm471"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4654-4661.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.02.010"},{"key":"e_1_3_2_8_2","unstructured":"Comit\u00e9 de l'Antibiogramme de la Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie. 2008. Communiqu\u00e9 2008. http:\/\/www.sfm.asso.fr\/publi\/general.php?pa=5\n."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.1128-1131.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.4.1249-1255.2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3003-3007.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.3.660-663.2001"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.11.012"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.3.1365-1373.1981"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00663-06"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(73)80154-1"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4485-4491.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401336200"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.5.626"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00818-07"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.4.891-897.2000"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.4026-4028.2002"},{"key":"e_1_3_2_24_2","first-page":"59","volume":"61","year":"2008","unstructured":"Psichogiou, M., P. T. Tassios, A. Avlamis, I. Stefanou, C. Kosmidis, E. Platsouka, O. Paniara, A. Xanthaki, M. Toutouza, G. L. Daikos, and L. S. Tzouvelekis. 2008. Ongoing epidemic of blaVIM-1-positive Klebsiella pneumoniae in Athens, Greece: a prospective survey. J. Antimicrob. Chemother.61:59-63.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00001-07"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.5.1229-1235.2000"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4443-4447.2005"},{"key":"e_1_3_2_28_2","first-page":"1659","year":"1989","unstructured":"Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, p. 1659, 2nd ed., vol. 1. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.","journal-title":"Molecular cloning: a laboratory manual,"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00166-08"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01337-07"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1086\/528719"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01573-07"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf210"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.306-325.2005"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00017-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00017-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:16:59Z","timestamp":1645474619000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00017-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1128\/AAC.00017-09"],"URL":"https:\/\/doi.org\/10.1128\/aac.00017-09","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1]]},"assertion":[{"value":"2009-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-11-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}