{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:03:57Z","timestamp":1792789437162,"version":"4.5.0"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We compared the affinities of ceftaroline for all penicillin-binding proteins (PBPs) with those of ceftriaxone and cefotaxime in 6\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            and 7\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            isolates with various resistance phenotypes. Ceftaroline MICs were \u22641 \u03bcg\/ml against all\n            <jats:italic>S. aureus<\/jats:italic>\n            isolates and were \u22640.25 \u03bcg\/ml for 4 of 7 isolates of\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            . Ceftaroline affinities for penicillin-susceptible\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            strains were in the order PBP2X and -3 &gt; PBP1A, -1B, and -2A &gt; PBP2B, and ceftaroline had \u22654-fold higher 50% inhibitory concentrations (IC\n            <jats:sub>50<\/jats:sub>\n            s) (0.1 to 4 \u03bcg\/ml) for PBP2X, -2A, -2B, and -3 than those for the other cephalosporins tested. Among 3 penicillin-resistant\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            strains, ceftaroline had a high affinity for PBP2X (IC\n            <jats:sub>50<\/jats:sub>\n            , 0.1 to 1 \u03bcg\/ml), a primary target for cephalosporin PBP binding activity, and high affinities for PBP2B (IC\n            <jats:sub>50<\/jats:sub>\n            , 0.5 to 4 \u03bcg\/ml) and PBP1A (IC\n            <jats:sub>50<\/jats:sub>\n            , 0.125 to 0.25 \u03bcg\/ml) as well, both of which are also known as major targets for PBP binding activity of cephalosporins. Ceftaroline PBP affinities in methicillin-susceptible\n            <jats:italic>S. aureus<\/jats:italic>\n            strains were greater than or equal to those of the 3 other \u03b2-lactams tested. Ceftaroline bound to PBP2a in methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            (IC\n            <jats:sub>50<\/jats:sub>\n            , 0.01 to 1 \u03bcg\/ml) with up to 256-fold-higher affinity than those of other agents. Ceftaroline demonstrated very good PBP affinity against all\n            <jats:italic>S. aureus<\/jats:italic>\n            and\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            strains tested, including resistant isolates.\n          <\/jats:p>","DOI":"10.1128\/aac.00019-10","type":"journal-article","created":{"date-parts":[[2010,3,2]],"date-time":"2010-03-02T04:54:26Z","timestamp":1267505666000},"page":"1670-1677","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":161,"title":["Affinity of Ceftaroline and Other \u03b2-Lactams for Penicillin-Binding Proteins from\n            <i>Staphylococcus aureus<\/i>\n            and\n            <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1128","volume":"54","author":[{"given":"K.","family":"Kosowska-Shick","sequence":"first","affiliation":[{"name":"Department of Pathology, Hershey Medical Center, Hershey, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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