{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T12:53:44Z","timestamp":1720961624458},"reference-count":41,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antibiotic resistance in\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            continues to be a major concern in public health. Resistance of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            bacteria to penicillin G is widespread in most developed countries, which has necessitated a change to newer drugs for treatment of gonococcal infections. Recent reports indicate that resistance to these newer drugs is increasing, highlighting the need for accurate therapeutic recommendations. In some countries or communities, however,\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates are still susceptible to penicillin, so the use of this antibiotic for single-dose treatments of medically under-resourced patients is beneficial. In order to evaluate the adequacy and sustainability of this treatment approach, we explored the presence and prevalence of chromosomally mediated resistance determinants in\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates collected from 2005 to 2007 in New Caledonia. We developed two new real-time PCR assays targeting the\n            <jats:italic>penB<\/jats:italic>\n            and\n            <jats:italic>mtrR<\/jats:italic>\n            determinants, to be used together with a previously described duplex assay targeting the\n            <jats:italic>penA<\/jats:italic>\n            and\n            <jats:italic>ponA<\/jats:italic>\n            determinants. The results of this study provided evidence that neither the most-common\n            <jats:italic>mtrR<\/jats:italic>\n            determinants nor the most-resistance-associated\n            <jats:italic>penB<\/jats:italic>\n            alleles are currently circulating in New Caledonia, suggesting that penicillin should still be considered a valuable treatment strategy. Additionally, using our genotyping assay, we observed an unexpected\n            <jats:italic>penB<\/jats:italic>\n            genotype at a relatively high frequency that was associated with a decreased susceptibility to penicillin (average MIC, 0.15 \u03bcg\/ml). Sequencing revealed that this genotype corresponded to an A102S mutation in the\n            <jats:italic>penB<\/jats:italic>\n            gene. The molecular tools developed in this study can be used successfully for prospective epidemiological monitoring and surveillance of penicillin susceptibility.\n          <\/jats:p>","DOI":"10.1128\/aac.00020-08","type":"journal-article","created":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:34:56Z","timestamp":1214872496000},"page":"3293-3300","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Genotyping as a Tool for Antibiotic Resistance Surveillance of\n            <i>Neisseria gonorrhoeae<\/i>\n            in New Caledonia: Evidence of a Novel Genotype Associated with Reduced Penicillin Susceptibility"],"prefix":"10.1128","volume":"52","author":[{"given":"Fre\u0301de\u0301rique","family":"Vernel-Pauillac","sequence":"first","affiliation":[{"name":"Institut Pasteur de Nouvelle-Cale\u0301donie, Laboratoire de Recherche en Bacte\u0301riologie, Noume\u0301a Cedex, New Caledonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sobhan","family":"Nandi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7365"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Nicholas","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7365"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cyrille","family":"Goarant","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Nouvelle-Cale\u0301donie, Laboratoire de Recherche en Bacte\u0301riologie, Noume\u0301a Cedex, New Caledonia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.03.004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.8.2169-2172.1998"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.38.100184.000551"},{"key":"e_1_3_2_5_2","first-page":"332","volume":"56","year":"2007","unstructured":"Centers for Disease Control and Prevention. 2007. 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