{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:44:47Z","timestamp":1760784287717,"version":"3.41.2"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000199","name":"USDA","doi-asserted-by":"crossref","award":["2014-06183","2014-67015-22106"],"award-info":[{"award-number":["2014-06183","2014-67015-22106"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI089441","R01AI111341","R01HD080670"],"award-info":[{"award-number":["R01AI089441","R01AI111341","R01HD080670"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005624","name":"PATH","doi-asserted-by":"publisher","award":["DFI 1850-02-405099"],"award-info":[{"award-number":["DFI 1850-02-405099"]}],"id":[{"id":"10.13039\/100005624","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Cryptosporidium parvum<\/jats:named-content>\n            calcium-dependent protein kinase 1 (\n            <jats:italic>Cp<\/jats:italic>\n            CDPK1) is a promising target for drug development against cryptosporidiosis. We report a series of low-nanomolar\n            <jats:italic>Cp<\/jats:italic>\n            CDPK1 5-aminopyrazole-4-carboxamide (AC) scaffold inhibitors that also potently inhibit\n            <jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>\n            growth\n            <jats:italic>in vitro<\/jats:italic>\n            . Correlation between anti-\n            <jats:italic>Cp<\/jats:italic>\n            CDPK1 and\n            <jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>\n            growth inhibition, as previously reported for pyrazolopyrimidines, was not apparent. Nonetheless, lead AC compounds exhibited a substantial reduction of parasite burden in the neonatal mouse cryptosporidiosis model when dosed at 25 mg\/kg.\n          <\/jats:p>","DOI":"10.1128\/aac.00020-17","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:31:13Z","timestamp":1495499473000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["5-Aminopyrazole-4-Carboxamide-Based Compounds Prevent the Growth of Cryptosporidium parvum"],"prefix":"10.1128","volume":"61","author":[{"given":"Wenlin","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Infectious Diseases, Center for Emerging &amp; Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew A.","family":"Hulverson","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Infectious Diseases, Center for Emerging &amp; Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongsheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly C.","family":"McCloskey","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Infectious Diseases, Center for Emerging &amp; Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah A.","family":"Schaefer","sequence":"additional","affiliation":[{"name":"School of Animal and Comparative Biomedical Sciences, College of Agriculture and Life Sciences, University of Arizona, Tucson, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant R.","family":"Whitman","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Infectious Diseases, Center for Emerging &amp; Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynn K.","family":"Barrett","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Infectious Diseases, Center for Emerging &amp; Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rama Subba Rao","family":"Vidadala","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael W.","family":"Riggs","sequence":"additional","affiliation":[{"name":"School of Animal and Comparative Biomedical Sciences, College of Agriculture and Life Sciences, University of Arizona, Tucson, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dustin J.","family":"Maly","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Washington, Seattle, Washington, USA"},{"name":"Department of Chemistry, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wesley C.","family":"Van Voorhis","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Infectious Diseases, Center for Emerging &amp; Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayode K.","family":"Ojo","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Infectious Diseases, Center for Emerging &amp; Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erkang","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70772-8"},{"key":"e_1_3_3_3_2","volume-title":"Mandell, Douglas, and Bennett's principles and practice of infectious diseases","author":"White AC","year":"2015","unstructured":"White AC . 2015. Cryptosporidium species. InBennettJE DolinR BlaserMJ (ed), Mandell, Douglas, and Bennett's principles and practice of infectious diseases, 8th ed. Elsevier\/Saunders, Philadelphia, PA.","edition":"8"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e328357e569"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60844-2"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199503303321304"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.6806-6816.2004"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ml100096t"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ml400315s"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw488"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit327"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw481"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.5b00319"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2016.10.014"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix120"},{"key":"e_1_3_3_16_2","volume-title":"Pharmacology","author":"Webster CRL","year":"2001","unstructured":"Webster CRL . 2001. Pharmacology. Teton NewMedia, Jackson Hole, WY."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00754-09"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00020-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00020-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:08:26Z","timestamp":1645477706000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00020-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1128\/AAC.00020-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.00020-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"type":"print","value":"0066-4804"},{"type":"electronic","value":"1098-6596"}],"subject":[],"published":{"date-parts":[[2017,8]]},"assertion":[{"value":"2017-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-05-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-07-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00020-17"}}