{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:17:06Z","timestamp":1788369426236,"version":"build-2803163510"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"crossref","award":["RS-2026-25611355"],"award-info":[{"award-number":["RS-2026-25611355"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["RS-2025-00513239"],"award-info":[{"award-number":["RS-2025-00513239"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["RS-2025-00553409"],"award-info":[{"award-number":["RS-2025-00553409"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The emergence of multidrug-resistant\n                      <jats:italic toggle=\"yes\">Candidozyma auris<\/jats:italic>\n                      has created an urgent need for antifungals with novel mechanisms of action. We screened 121 nucleoside and nucleobase derivatives against\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      and other clinically relevant\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      species to identify effective antimetabolite scaffolds. Among all compounds tested, only the C-5-fluorinated nucleosides 5-fluorouridine (5-FU) and 5-fluorocytidine exhibited potent antifungal activity. 5-FU demonstrated consistently low minimum inhibitory concentrations (0.5\u201310 \u00b5g\/mL) across multiple\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      species, including fluconazole-resistant strains, and displayed fungicidal activity against\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      at concentrations lower than several frontline antifungals. Uridine rescue assays confirmed competitive interference with endogenous uridine metabolism. Checkerboard assays revealed synergistic or additive interactions between 5-FU and clinically used azoles and echinocandins. Toxicity evaluation using plant, nematode, and human cell models showed minimal cytotoxicity even at concentrations far exceeding antifungal minimum inhibitory concentrations.\n                      <jats:italic toggle=\"yes\">In silico<\/jats:italic>\n                      docking analyses revealed dual inhibition of thymidylate synthase and RNA polymerase III, explaining the fungicidal phenotype and broad-spectrum efficacy. These findings identify 5-fluorouridine as a promising antifungal candidate and establish C-5-halogenated nucleosides as a privileged scaffold for antifungal drug development.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00027-26","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T13:46:01Z","timestamp":1786715161000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Antifungal activity of the nucleoside mimetic 5-fluorouridine against\n                    <i>Candidozyma auris<\/i>\n                    and\n                    <i>Candida<\/i>\n                    species"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8519-5297","authenticated-orcid":false,"given":"Bharath Reddy","family":"Boya","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05yc6p159","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemical Engineering, Yeungnam University","place":["Gyeongsan, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezhaveni","family":"Sathiyamoorthi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05yc6p159","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemical Engineering, Yeungnam University","place":["Gyeongsan, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Hyung","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05yc6p159","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemical Engineering, Yeungnam University","place":["Gyeongsan, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1383-1682","authenticated-orcid":true,"given":"Jintae","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05yc6p159","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemical Engineering, Yeungnam University","place":["Gyeongsan, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myab053"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2018.26"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/diseases13040093"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.15698\/mic2020.06.718"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9040807"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.adf8972"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02475-23"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00952"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.armc.2021.09.001"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10071299"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/15376516.2025.2559286"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.382"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011066"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbaf347"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1124\/pharmrev.123.000863"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2009.954511"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-023-12884-8"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2025.117528"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01737-23"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.5c04793"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00287-24"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.163091"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms262210836"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2026.121602"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2022.101537"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2025.119547"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3357"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/narcan\/zcab032"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2025.1662442"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.3906\/kim-1808-34"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.3897\/pharmacia.68.e56543"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0008-6215(00)00089-6"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29102390"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0959-8049(98)00058-6"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2024.101778"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.5.1795"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.12.6215-6216.2005"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1279250"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8344"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm201506e"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules13081551"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof10100698"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2019.04.003"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-03533-3"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.09.128"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.24338"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-72467-1_2"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201800279"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.3390\/medicina59061107"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15370"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6515-1_21"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00027-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00027-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T16:54:15Z","timestamp":1788368055000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.00027-26"}},"subtitle":[],"editor":[{"given":"Andreas H.","family":"Groll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":51,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,2]]}},"alternative-id":["10.1128\/aac.00027-26"],"URL":"https:\/\/doi.org\/10.1128\/aac.00027-26","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]},"assertion":[{"value":"2026-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00027-26"}}