{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T08:15:19Z","timestamp":1761293719181,"version":"3.41.2"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"University Paris XI\/INSERM"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Integrons are genetic elements that can acquire and rearrange gene cassettes. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>BEL-1<\/jats:sub>\n            gene encodes an extended-spectrum \u03b2-lactamase, BEL-1, that is present at the second position of the variable region of class 1 integrons identified in\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n            . The mobility of the\n            <jats:italic>bel-1<\/jats:italic>\n            gene cassette was analyzed under physiological conditions and with the integrase gene being overexpressed. Cassette mobility in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            was detected by excision\/integration into the recipient integron In\n            <jats:italic>3<\/jats:italic>\n            on the conjugative plasmid R388 with the overproduced integrase. Despite several antibiotic pressures, the\n            <jats:italic>bel-1<\/jats:italic>\n            cassette remained at the second position in the integron, highlighting its stability in\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            . Overexpression of the integrase gene in\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            induced\n            <jats:italic>bel-1<\/jats:italic>\n            cassette recombination. However, cassettes containing two genes (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>BEL-1<\/jats:sub>\n            and\n            <jats:italic>smr2<\/jats:italic>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>BEL-1<\/jats:sub>\n            and\n            <jats:italic>aacA4<\/jats:italic>\n            ) were excised, suggesting that the\n            <jats:italic>bel-1<\/jats:italic>\n            cassette\n            <jats:italic>attC<\/jats:italic>\n            site was defective. We show that\n            <jats:italic>bel-1<\/jats:italic>\n            is a stable gene cassette under physiological growth conditions, irrespective of the selective antibiotic pressure, that may be mobilized upon overexpression of the integrase gene.\n          <\/jats:p>","DOI":"10.1128\/aac.00030-18","type":"journal-article","created":{"date-parts":[[2018,3,28]],"date-time":"2018-03-28T16:14:14Z","timestamp":1522253654000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Integrase-Mediated Recombination of the\n            <i>bel-1<\/i>\n            Gene Cassette Encoding the Extended-Spectrum \u03b2-Lactamase BEL-1"],"prefix":"10.1128","volume":"62","author":[{"given":"Nacim","family":"Bouheraoua","sequence":"first","affiliation":[{"name":"Quinze-Vingts National Ophthalmology Hospital, UPMC-Sorbonne Universities, Paris, 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Reference Center for Antibiotic Resistance Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9my","family":"Bonnin","sequence":"additional","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"},{"name":"EERA (Evolution and Ecology of Resistance to Antibiotics) Unit, Institut Pasteur-APHP-Universit\u00e9 Paris Sud, Paris, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Laroche","sequence":"additional","affiliation":[{"name":"Quinze-Vingts National Ophthalmology Hospital, UPMC-Sorbonne Universities, Paris, 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