{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:47:03Z","timestamp":1785851223093,"version":"3.56.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"crossref","award":["2020\/39\/O\/NZ7\/00145"],"award-info":[{"award-number":["2020\/39\/O\/NZ7\/00145"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                      (CRAb) causes hard-to-control healthcare outbreaks, but the mobile resistance determinants that sustain regional spread are often underrecognized. We characterized 70 CRAb isolates from 11 hospitals in southern Poland (2019\u20132022) using whole-genome sequencing and core genome multilocus sequence typing (cgMLST), with reconstruction of carbapenemase context and plasmid structure. All isolates belonged to international clone 2 (IC2; ST2, Pasteur scheme) or to ST425 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 47), ST195 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 19), ST208 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 2), and ST348 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 1) according to the Oxford scheme.\n                      <jats:italic toggle=\"yes\">In silico<\/jats:italic>\n                      KL\/OCL typing further differentiated the collection. All isolates were OCL18, whereas the K-locus distribution was dominated by KL125 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 48) and KL3 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 19), with two KL2 isolates and one KL9 isolate.\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-72<\/jats:sub>\n                      was detected in 50\/70 isolates (71.4%), indicating an atypically high regional dominance. The OXA-72-encoding gene was carried on the same ~10.9 kb GR2\/repAci1 plasmid (p398AB) in all isolates, which was sequence-identical across centers and contained two oppositely oriented copies of\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-72<\/jats:sub>\n                      . Transformation of p398AB into susceptible\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      ATCC17978 conferred a carbapenem-resistant phenotype. cgMLST identified a large multicenter cluster of\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-72<\/jats:sub>\n                      -positive isolates spanning multiple cities and hospitals, supporting inter-facility dissemination of a successful IC2 lineage, together with a conserved resistance plasmid. The strict conservation of p398AB during regional spread provides a practical target for rapid molecular screening in outbreak investigations. In contrast,\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-23<\/jats:sub>\n                      and a novel\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-23<\/jats:sub>\n                      -like variant (\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-1223<\/jats:sub>\n                      ) were confined to a minority of isolates. Overall, these data highlight an unusual dominance of plasmid-encoded\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-72<\/jats:sub>\n                      in a multicenter IC2 outbreak and underscore the need for surveillance integrating clonal relatedness with tracking of transferable resistance plasmids.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00039-26","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T13:58:00Z","timestamp":1782482280000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Multicenter outbreak of plasmid-encoded\n                    <i>bla<\/i>\n                    <sub>OXA-72<\/sub>\n                    <i>Acinetobacter baumannii<\/i>\n                    in southern Poland"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4254-2804","authenticated-orcid":true,"given":"T.","family":"Kasperski","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Jagiellonian University Medical College","place":["Krakow, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4592-5742","authenticated-orcid":false,"given":"R. Arazo","family":"del Pino","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05mxhda18","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne","place":["Cologne, Germany"]},{"id":[{"id":"https:\/\/ror.org\/028s4q594","id-type":"ROR","asserted-by":"publisher"}],"name":"German Center for Infection Research (DZIF), Partner Site Bonn-Cologne","place":["Cologne, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8591-8184","authenticated-orcid":true,"given":"K.","family":"Xanthopoulou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05mxhda18","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne","place":["Cologne, Germany"]},{"id":[{"id":"https:\/\/ror.org\/028s4q594","id-type":"ROR","asserted-by":"publisher"}],"name":"German Center for Infection Research (DZIF), Partner Site Bonn-Cologne","place":["Cologne, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4910-4655","authenticated-orcid":false,"given":"T.","family":"Burgwinkel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05mxhda18","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne","place":["Cologne, Germany"]},{"id":[{"id":"https:\/\/ror.org\/028s4q594","id-type":"ROR","asserted-by":"publisher"}],"name":"German Center for Infection Research (DZIF), Partner Site Bonn-Cologne","place":["Cologne, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Pomorska-Weso\u0142owska","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Analytical and Microbiological Laboratory of Ruda Slaska, KORLAB NZOZ","place":["Ruda Slaska, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0756-1639","authenticated-orcid":true,"given":"J.","family":"W\u00f3jkowska-Mach","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Jagiellonian University Medical College","place":["Krakow, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0814-8638","authenticated-orcid":true,"given":"A.","family":"Chmielarczyk","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Jagiellonian University Medical College","place":["Krakow, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8677-9454","authenticated-orcid":false,"given":"P. 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