{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:52:03Z","timestamp":1791287523169,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2008,8,1]],"date-time":"2008-08-01T00:00:00Z","timestamp":1217548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Although antimicrobial therapy of leptospirosis has been studied in a few randomized controlled clinical studies, those studies were limited to specific regions of the world and few have characterized infecting strains. A broth microdilution technique for the assessment of antibiotic susceptibility has been developed at Brooke Army Medical Center. In the present study, we assessed the susceptibilities of 13<jats:italic>Leptospira<\/jats:italic>isolates (including recent clinical isolates) from Egypt, Thailand, Nicaragua, and Hawaii to 13 antimicrobial agents. Ampicillin, cefepime, azithromycin, and clarithromycin were found to have MICs below the lower limit of detection (0.016 \u03bcg\/ml). Cefotaxime, ceftriaxone, imipenem-cilastatin, penicillin G, moxifloxacin, ciprofloxacin, and levofloxacin had MIC<jats:sub>90<\/jats:sub>s between 0.030 and 0.125 \u03bcg\/ml. Doxycycline and tetracycline had the highest MIC<jats:sub>90<\/jats:sub>s: 2 and 4 \u03bcg\/ml, respectively. Doxycycline and tetracycline were noted to have slightly higher MICs against isolates from Egypt than against strains from Thailand or Hawaii; otherwise, the susceptibility patterns were similar. There appears to be possible variability in susceptibility to some antimicrobial agents among strains, suggesting that more extensive testing to look for geographic variability should be pursued.<\/jats:p>","DOI":"10.1128\/aac.00044-08","type":"journal-article","created":{"date-parts":[[2008,4,15]],"date-time":"2008-04-15T00:46:01Z","timestamp":1208220361000},"page":"2750-2754","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Antimicrobial Susceptibilities of Geographically Diverse Clinical Human Isolates of<i>Leptospira<\/i>"],"prefix":"10.1128","volume":"52","author":[{"given":"Roseanne A.","family":"Ressner","sequence":"first","affiliation":[{"name":"Brooke Army Medical Center, Fort Sam Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew E.","family":"Griffith","sequence":"additional","affiliation":[{"name":"Brooke Army Medical Center, Fort Sam Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam L.","family":"Beckius","sequence":"additional","affiliation":[{"name":"Brooke Army Medical Center, Fort Sam Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Pimentel","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit 3, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Scott","family":"Miller","sequence":"additional","affiliation":[{"name":"Walter Reed Army Institute of Research, Washington, DC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Mende","sequence":"additional","affiliation":[{"name":"Infectious Disease Clinical Research Program, Fort Sam Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan L.","family":"Fraser","sequence":"additional","affiliation":[{"name":"Walter Reed Army Medical Center, Washington, DC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renee L.","family":"Galloway","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duane R.","family":"Hospenthal","sequence":"additional","affiliation":[{"name":"Brooke Army Medical Center, Fort Sam Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinton K.","family":"Murray","sequence":"additional","affiliation":[{"name":"Brooke Army Medical Center, Fort Sam Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.6.835"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2460\/ajvr.1996.57.01.59","volume":"57","year":"1996","unstructured":"Alt, D. 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