{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:51:44Z","timestamp":1793235104001,"version":"4.5.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"US Department of Defense Health Program","award":["WUN D1430"],"award-info":[{"award-number":["WUN D1430"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,9,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n The emergence and spread of chloroquine-resistant\n Plasmodium vivax<\/jats:italic>\n have necessitated the assessment of alternative blood schizonticidal drugs. In Vietnam, chloroquine-resistant\n P. vivax<\/jats:italic>\n malaria has been reported. In an open-label, single-arm trial, the safety, tolerability, and efficacy of pyronaridine-artesunate (Pyramax, PA) was evaluated in Dak Nong province, Vietnam. A 3-day course of PA was administered to adults and children (\u226520 kg) infected with\n P. vivax<\/jats:italic>\n . Patients also received primaquine (0.25 mg\/kg daily for 14 days). PA was well tolerated with transient asymptomatic increases in liver transaminases. The per-protocol proportion of patients with day 42 PCR-unadjusted adequate clinical and parasitological response was 96.0% (95% CI, 84.9%\u201399.0%,\n n<\/jats:italic>\n = 48\/50). The median parasite clearance time was 12 h (range, 12\u201336 h), with a median fever clearance time of 24 h (range, 12\u201360 h). Single nucleotide polymorphisms (SNPs) as potential genetic markers of reduced drug susceptibility were analyzed in three putative drug resistance markers,\n Pvcrt-o<\/jats:italic>\n ,\n Pvmdr1<\/jats:italic>\n , and\n PvK12<\/jats:italic>\n . Insertion at position K10 of the\n Pvcrt-o<\/jats:italic>\n gene was found in 74.6% (44\/59) of isolates.\n Pvmdr1<\/jats:italic>\n SNPs at Y976F and F1076L were present in 61% (36\/59) and 78% (46\/59), respectively. Amplification of\n Pvmdr1<\/jats:italic>\n gene (two copies) was found in 5.1% (3\/59) of parasite samples. Only 5.1% (3\/59) of isolates had mutation 552I of the\n PvK12<\/jats:italic>\n gene. Overall, PA rapidly cleared\n P. vivax<\/jats:italic>\n blood asexual stages and was highly efficacious in treating vivax malaria, with no evidence of artemisinin resistance found. PA provides an alternative to chloroquine treatment for vivax malaria in Vietnam.\n <\/jats:p>\n <\/jats:sec>\n \n CLINICAL TRIALS<\/jats:title>\n \n This study is registered with the\n \n Australian New Zealand Clinical Trials Registry<\/jats:ext-link>\n as\n ACTRN12618001429246<\/jats:ext-link>\n <\/jats:related-object>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/aac.00044-24","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T13:02:02Z","timestamp":1721826122000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Therapeutic efficacy of pyronaridine-artesunate (Pyramax) in treating\n Plasmodium vivax<\/i>\n malaria in the central highlands of Vietnam"],"prefix":"10.1128","volume":"68","author":[{"given":"Nguyen Duc","family":"Manh","sequence":"first","affiliation":[{"name":"Vietnam People\u2019s Army Military Institute of Preventive Medicine, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Van","family":"Thanh","sequence":"additional","affiliation":[{"name":"Vietnam People\u2019s Army Military Institute of Preventive Medicine, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huynh Hong","family":"Quang","sequence":"additional","affiliation":[{"name":"Vietnam Ministry of Health Institute of Malariology, Parasitology and Entomology, Qui Nhon, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thi Thanh","family":"Van","sequence":"additional","affiliation":[{"name":"Vietnam People\u2019s Army Military Institute of Preventive Medicine, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Ngoc","family":"San","sequence":"additional","affiliation":[{"name":"Vietnam People\u2019s Army Military Institute of Preventive Medicine, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguen Chinh","family":"Phong","sequence":"additional","affiliation":[{"name":"Vietnam People\u2019s Army Military Institute of Preventive Medicine, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey W.","family":"Birrell","sequence":"additional","affiliation":[{"name":"Australian Defense Force Malaria and Infectious Disease Institute, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly A.","family":"Edgel","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit INDO PACIFIC, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J.","family":"Martin","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit INDO PACIFIC, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Edstein","sequence":"additional","affiliation":[{"name":"Australian Defense Force Malaria and Infectious Disease Institute, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8142-8468","authenticated-orcid":false,"given":"Marina","family":"Chavchich","sequence":"additional","affiliation":[{"name":"Australian Defense Force Malaria and Infectious Disease Institute, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050127"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2009.02.003"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-11-12"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30596-6"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0141"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2020.03.009"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(89)91792-3"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.11.4075-4083.2004"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70855-2"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2017.03.003"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70079-7"},{"key":"e_1_3_4_14_2","volume-title":"Guidelines for the treatment of malaria","author":"WHO","year":"2015","unstructured":"WHO. 2015. Guidelines for the treatment of malaria. 3rd edition. Geneva, Switzerland.","edition":"3"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD008492.pub3"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2002.00948.x"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00791-15"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00095-21"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01940-16"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-11-355"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30391-3"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2021.04.002"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2020.12.001"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/426830"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001089"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1086\/592451"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01459-07"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12876"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03908-14"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-2749-3"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00276-21"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-10-339"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-008-0506-6"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00365-11"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014501"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01273-19"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.20-0185"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-022-04422-0"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-12-70"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1003669"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30291-5"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2003.69.14"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-10-297"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03829-14"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02004-15"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1301936"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60322-4"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1007125"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00039-16"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02242-18"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz580"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00092-1"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq249"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148172"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2010.08.011"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0434"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02362-14"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00044-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00044-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T15:07:10Z","timestamp":1726672030000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.00044-24"}},"subtitle":[],"editor":[{"given":"Audrey","family":"Odom John","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,9,4]]},"references-count":55,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9,4]]}},"alternative-id":["10.1128\/aac.00044-24"],"URL":"https:\/\/doi.org\/10.1128\/aac.00044-24","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,4]]},"assertion":[{"value":"2024-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00044-24"}}