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An<jats:italic>F. nucleatum<\/jats:italic>subsp.<jats:italic>polymorphum<\/jats:italic>strain resistant to penicillin and amoxicillin was isolated from a human periodontitis sample. DNA cloning and sequencing revealed a 765-bp open reading frame encoding a new class D \u03b2-lactamase named FUS-1 (OXA-85). A recombinant<jats:italic>Escherichia coli<\/jats:italic>strain carrying the<jats:italic>bla<\/jats:italic><jats:sub>FUS-1<\/jats:sub>gene exhibited resistance to amoxicillin with a moderate decrease in the MICs with clavulanic acid. The<jats:italic>bla<\/jats:italic><jats:sub>FUS-1<\/jats:sub>gene was found in two additional clonally unrelated<jats:italic>F. nucleatum<\/jats:italic>subsp.<jats:italic>polymorphum<\/jats:italic>isolates. It was located on the chromosome in a peculiar genetic environment where a gene encoding a putative transposase-like protein is found, suggesting a possible acquisition of this class D \u03b2-lactamase gene. The FUS-1 enzyme showed the closest ancestral relationship with OXA-63 from<jats:italic>Brachyspira pilosicoli<\/jats:italic>(53% identity) and with putative chromosomal \u03b2-lactamases of<jats:italic>Campylobacter<\/jats:italic>spp. (40 to 42% identity). FUS-1 presents all of the conserved structural motifs of class D \u03b2-lactamases. Kinetic analysis revealed that FUS-1 exhibits a narrow substrate profile, efficiently hydrolyzing benzylpenicillin and oxacillin. FUS-1 was poorly inactivated by clavulanate and NaCl. FUS-1 is the first example of a class D \u03b2-lactamase produced by a gram-negative, anaerobic, rod-shaped bacterium to be characterized.<\/jats:p>","DOI":"10.1128\/aac.00058-06","type":"journal-article","created":{"date-parts":[[2006,7,26]],"date-time":"2006-07-26T18:17:58Z","timestamp":1153937878000},"page":"2673-2679","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Genetic and Biochemical Characterization of FUS-1 (OXA-85), a Narrow-Spectrum Class D \u03b2-Lactamase from<i>Fusobacterium nucleatum<\/i>subsp.<i>polymorphum<\/i>"],"prefix":"10.1128","volume":"50","author":[{"given":"Christine","family":"Voha","sequence":"first","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie et Epide\u0301miologie Mole\u0301culaire, Universite\u0301 de Nice, Centre Hospitalier Universitaire de Nice, F-06202 Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Denis","family":"Docquier","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines et Laboratoire d'Enzymologie, Universite\u0301 de Lie\u0300ge, B-4000 Sart-Tilman, Belgium"},{"name":"Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Universita\u0300 di Siena, I-53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Universita\u0300 di Siena, I-53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Fosse","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie et Epide\u0301miologie Mole\u0301culaire, Universite\u0301 de Nice, Centre Hospitalier Universitaire de Nice, F-06202 Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.6.2515-2518.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.8.1546"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-002-2328-y"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2354-2357.2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2003.00717.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-40-1-74"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.5.611"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0902-0055.2001.00096.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3509-3516.2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3144-3149.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-2-296"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.2043-2048.2004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.1973-1980.2005"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.4.797"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3140-3146.2000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.9.3940-3943.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.7.2005-2018.2002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.566003"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1270"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.7.1584"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2950871"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.8.2386-2389.2001"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"865","DOI":"10.2174\/1381612805666230112185101","volume":"5","year":"1999","unstructured":"Naas, T., and P. 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