{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T02:40:54Z","timestamp":1789267254716,"version":"build-2803163510"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study evaluated the\n            <jats:italic>in vitro<\/jats:italic>\n            activity of aminoglycoside antibiotics and tigecycline against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pythium insidiosum<\/jats:named-content>\n            . The susceptibility tests were carried out using the broth microdilution method in accordance with the CLSI document M38-A2. MIC values for gentamicin, neomycin, paromomycin, and streptomycin ranged from 32 to 64 mg\/liter, and the minimal fungicidal concentration (MFC) ranged from 32 to 128 mg\/liter, which are incompatible with safe concentrations of these drugs in plasma\n            <jats:italic>in vivo<\/jats:italic>\n            . Tigecycline showed the lowest MIC (0.25 to 2 mg\/liter) and MFC (1 to 8 mg\/liter) range values. The\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility observed to tigecycline makes this drug a good option in future tests\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            for the management of pythiosis.\n          <\/jats:p>","DOI":"10.1128\/aac.00073-12","type":"journal-article","created":{"date-parts":[[2012,4,17]],"date-time":"2012-04-17T05:22:29Z","timestamp":1334640149000},"page":"4021-4023","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["<i>In Vitro<\/i>\n            Susceptibility of Pythium insidiosum Isolates to Aminoglycoside Antibiotics and Tigecycline"],"prefix":"10.1128","volume":"56","author":[{"given":"Deise Luiza","family":"Mahl","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francielli Pantella Kunz","family":"de Jesus","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rico Silva","family":"Loreto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9gis Adriel","family":"Zanette","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laerte","family":"Ferreiro","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiara Ben","family":"Pilotto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sydney Hartz","family":"Alves","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janio Morais","family":"Santurio","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1093\/clinids\/24.5.796","article-title":"A meta-analysis of the relative efficacy and toxicity of single daily dosing versus multiple daily dosing of aminoglycosides","volume":"24","author":"Ali MZ","year":"1997","unstructured":"AliMZ GoetzMB. 1997. A meta-analysis of the relative efficacy and toxicity of single daily dosing versus multiple daily dosing of aminoglycosides. Clin. Infect. Dis. 24:796\u2013809.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_3_2","unstructured":"Clinical and Laboratory Standards Institute. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi: approved standard 2nd ed M38-A2. CLSI Wayne PA."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.07.019"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2002.tb02346.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1094\/Phyto-72-750","article-title":"Variability in growth of Phytophthora cinnamomi isolates in response to antibiotics","volume":"72","author":"Leary JV","year":"1982","unstructured":"LearyJV ZentmyerGA KlureLJ PondEC GranthamGL. 1982. 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Microbiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.2307\/3761492","article-title":"In vitro effect of streptomycin on clinical isolates of Pythium insidiosum","volume":"92","author":"McMeekin D","year":"2000","unstructured":"McMeekinD MendozaL. 2000. In vitro effect of streptomycin on clinical isolates of Pythium insidiosum. Mycologia 92:371\u2013373.","journal-title":"Mycologia"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.1.220-229.2005"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm332"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-108-1-133"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06126-11"},{"key":"e_1_3_2_15_2","first-page":"1","article-title":"Pythiosis: an emergent mycosis","volume":"34","author":"Santurio JM","year":"2006","unstructured":"SanturioJM AlvesSH PereiraDB ArgentaJS. 2006. Pythiosis: an emergent mycosis. Acta Sci. Vet. 34:1\u201314.","journal-title":"Acta Sci. 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