{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:38:06Z","timestamp":1779331086334,"version":"3.51.4"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Flaviviruses cause severe disease in humans and are a public health priority worldwide. However, no effective therapies or drugs are commercially available yet. Several flavivirus replicon-based assays amenable to high-throughput screening of inhibitors have been reported recently. We developed and performed a replicon-based high-throughput assay for screening small-molecule inhibitors of yellow fever virus (YFV) replication. This assay utilized packaged pseudoinfectious particles containing a YFV replicon that expresses\n            <jats:italic>Renilla<\/jats:italic>\n            luciferase in a replication-dependent manner. Several small-molecule compounds with inhibitory activity at micromolar concentrations were identified in the high-throughput screen. These compounds were subsequently tested for their inhibitory activities against YFV replication and propagation in low-throughput assays. Furthermore, YFV mutants that escaped inhibition by two of the compounds were isolated, and in both cases, the mutations were mapped to the NS4B coding region, suggesting a novel inhibitory target for these compounds. This study opens up new avenues for pursuing the nonenzymatic nonstructural proteins as targets for antivirals against YFV and other flaviviruses.\n          <\/jats:p>","DOI":"10.1128\/aac.00074-09","type":"journal-article","created":{"date-parts":[[2009,8,4]],"date-time":"2009-08-04T01:41:09Z","timestamp":1249350069000},"page":"4103-4114","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Identification of Inhibitors of Yellow Fever Virus Replication Using a Replicon-Based High-Throughput Assay"],"prefix":"10.1128","volume":"53","author":[{"given":"Chinmay G.","family":"Patkar","sequence":"first","affiliation":[{"name":"Markey Center for Structural Biology, Department of Biological Sciences, 915 W. State Street, Purdue University, West Lafayette, Indiana 47907-2054"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha","family":"Larsen","sequence":"additional","affiliation":[{"name":"Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, Michigan 48109-2216"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Owston","sequence":"additional","affiliation":[{"name":"Markey Center for Structural Biology, Department of Biological Sciences, 915 W. State Street, Purdue University, West Lafayette, Indiana 47907-2054"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet L.","family":"Smith","sequence":"additional","affiliation":[{"name":"Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, Michigan 48109-2216"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Markey Center for Structural Biology, Department of Biological Sciences, 915 W. 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