{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:18:39Z","timestamp":1791177519963,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Fatty acid biosynthesis is a promising novel antibiotic target. Two inhibitors of fatty acid biosynthesis, platencin and platensimycin, were recently discovered and their molecular targets identified. Numerous structure-activity relationship studies for both platencin and platensimycin are currently being undertaken. We established a proteomic signature for fatty acid biosynthesis inhibition in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Bacillus subtilis<\/jats:named-content>\n            using platencin, platensimycin, cerulenin, and triclosan. The induced proteins, FabHA, FabHB, FabF, FabI, PlsX, and PanB, are enzymes involved in fatty acid biosynthesis and thus linked directly to the target pathway. The proteomic signature can now be used to assess the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">in vivo<\/jats:named-content>\n            mechanisms of action of compounds derived from structure-activity relationship programs, as demonstrated for the platensimycin-inspired chromium bioorganometallic PM47. It will further serve as a reference signature for structurally novel natural and synthetic antimicrobial compounds with unknown mechanisms of action. In summary, we described a proteomic signature in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. subtilis<\/jats:named-content>\n            consisting of six upregulated proteins that is diagnostic of fatty acid biosynthesis inhibition and thus can be applied to advance antibacterial drug discovery programs.\n          <\/jats:p>","DOI":"10.1128\/aac.00078-11","type":"journal-article","created":{"date-parts":[[2011,3,8]],"date-time":"2011-03-08T07:04:54Z","timestamp":1299567894000},"page":"2590-2596","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Proteomic Signature of Fatty Acid Biosynthesis Inhibition Available for In Vivo Mechanism-of-Action Studies"],"prefix":"10.1128","volume":"55","author":[{"given":"Michaela","family":"Wenzel","sequence":"first","affiliation":[{"name":"Ruhr University Bochum, Biology of Microorganisms, Universit\u00e4tsstra\u00dfe 150, 44801 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malay","family":"Patra","sequence":"additional","affiliation":[{"name":"Ruhr University Bochum, Bioinorganic Chemistry, Universit\u00e4tsstra\u00dfe 150, 44801 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Albrecht","sequence":"additional","affiliation":[{"name":"Ernst Moritz Arndt University Greifswald, Institute for Microbiology, Friedrich-Ludwig-Jahn-Stra\u00dfe 15a, 17489 Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Y.-K.","family":"Chen","sequence":"additional","affiliation":[{"name":"Chemical Synthesis Laboratory@Biopolis, Institute of Chemical and Engineering Sciences (ICES), Agency for Science, Technology and Research (ASTAR), 11 Biopolis Way, The Helios Block, #03-08, Singapore 138667, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. C.","family":"Nicolaou","sequence":"additional","affiliation":[{"name":"Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, and Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils","family":"Metzler-Nolte","sequence":"additional","affiliation":[{"name":"Ruhr University Bochum, Bioinorganic Chemistry, Universit\u00e4tsstra\u00dfe 150, 44801 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia E.","family":"Bandow","sequence":"additional","affiliation":[{"name":"Ruhr University Bochum, Biology of Microorganisms, Universit\u00e4tsstra\u00dfe 150, 44801 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.81.5.741-746.1961"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.3.948-955.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200701184"},{"key":"e_1_3_2_5_2","first-page":"79","article-title":"Proteomic profiling of cellular stresses in Bacillus subtilis reveals cellular networks and assists in elucidating antibiotic mechanisms of action","volume":"64","author":"Bandow J. 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