{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:58:25Z","timestamp":1779343105504,"version":"3.51.4"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"the National Institute of Allergy and Infectious Diseases, National Institutes of Health","doi-asserted-by":"publisher","award":["K08AI112506"],"award-info":[{"award-number":["K08AI112506"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"the National Institute of Allergy and Infectious Diseases, National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI072219"],"award-info":[{"award-number":["R01AI072219"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"the National Institute of Allergy and Infectious Diseases, National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI063517"],"award-info":[{"award-number":["R01AI063517"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"the National Institute of Allergy and Infectious Diseases, National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI100560"],"award-info":[{"award-number":["R01AI100560"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n Multidrug-resistant (MDR)\n Enterobacteriaceae<\/jats:named-content>\n infections are increasing in U.S. children; however, there is a paucity of multicentered analyses of antibiotic resistance genes responsible for MDR phenotypes among pediatric\n Enterobacteriaceae<\/jats:named-content>\n isolates. In this study, 225 isolates phenotypically identified as extended-spectrum \u03b2-lactamase (ESBL) or carbapenemase producers, recovered from children ages 0 to 18 years hospitalized between January 2011 and April 2015 at three Chicago area hospitals, were analyzed. We used DNA microarray platforms to detect ESBL, plasmid-mediated AmpC (pAmpC), and carbapenemase type \u03b2-lactamase (\n bla<\/jats:italic>\n ) genes. Repetitive-sequence-based PCR and multilocus sequence typing (MLST) were performed to assess isolate similarity. Plasmid replicon typing was conducted to classify plasmids. The median patient age was 4.2 years, 56% were female, and 44% presented in the outpatient setting. The majority (60.9%) of isolates were\n Escherichia coli<\/jats:named-content>\n and from urinary sources (69.8%). Of 225 isolates exhibiting ESBL- or carbapenemase-producing phenotypes, 90.7% contained a\n bla<\/jats:italic>\n gene. The most common genotype was the\n bla<\/jats:italic>\n CTX-M-1<\/jats:sub>\n group (49.8%); 1.8% were carbapenem-resistant\n Enterobacteriaceae<\/jats:named-content>\n (three\n bla<\/jats:italic>\n KPC<\/jats:sub>\n and one\n bla<\/jats:italic>\n IMP<\/jats:sub>\n ). Overall, pAmpC (\n bla<\/jats:italic>\n ACT\/MIR<\/jats:sub>\n and\n bla<\/jats:italic>\n CMY<\/jats:sub>\n ) were present in 14.2%. The predominant\n E. coli<\/jats:named-content>\n phylogenetic group was the virulent B2 group (67.6%) associated with ST43\/ST131 (Pasteur\/Achtman MLST scheme) containing the\n bla<\/jats:italic>\n CTX-M-1<\/jats:sub>\n group (84%), and plasmid replicon types FIA, FII, and FIB.\n K. pneumoniae<\/jats:named-content>\n harboring\n bla<\/jats:italic>\n KPC<\/jats:sub>\n were non-ST258 with replicon types I1 and A\/C.\n Enterobacter<\/jats:named-content>\n spp. carrying\n bla<\/jats:italic>\n ACT\/MIR<\/jats:sub>\n contained plasmid replicon FIIA. We found that \u03b2-lactam resistance in children is diverse and that certain resistance mechanisms differ from known circulating genotypes in adults in an endemic area. The potential impact of complex molecular types and the silent dissemination of MDR\n Enterobacteriaceae<\/jats:named-content>\n in a vulnerable population needs to be studied further.\n <\/jats:p>","DOI":"10.1128\/aac.00098-16","type":"journal-article","created":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T02:23:38Z","timestamp":1459218218000},"page":"3462-3469","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Analysis of \u03b2-Lactamase Resistance Determinants in Enterobacteriaceae from Chicago Children: a Multicenter Survey"],"prefix":"10.1128","volume":"60","author":[{"given":"Latania K.","family":"Logan","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Rush University Medical Center, Chicago, Illinois, USA"},{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea M.","family":"Hujer","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"},{"name":"Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven H.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. Nicholas","family":"Domitrovic","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"},{"name":"Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan D.","family":"Rudin","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"},{"name":"Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaotian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ann and Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois, USA"},{"name":"Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia K.","family":"Qureshi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Loyola University Medical Center, Maywood, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary K.","family":"Hayden","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Pathology, Rush University Medical Center, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felicia A.","family":"Scaggs","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Rush University Medical Center, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anand","family":"Karadkhele","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Rush University Medical Center, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"},{"name":"Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"},{"name":"Department of Pharmacology, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"},{"name":"Departments of Molecular Biology and Microbiology, Case Western Reserve School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.08.011"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01009-09"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2006.05.238"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102911"},{"key":"e_1_3_3_6_2","volume-title":"54th Intersci Conf Antimicrob Agents Chemother (ICAAC)","author":"Bush K","year":"2014","unstructured":"Bush K. 2014. ICAAC Award Lecture: \u03b2-lactamases: ubiquitous and formidable. 54th Intersci Conf Antimicrob Agents Chemother (ICAAC). 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