{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:16:14Z","timestamp":1768518974812,"version":"3.49.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In the absence of an effective vaccine against HIV-1 infection, anti-HIV-1 strategies play a major role in disease control. However, the rapid emergence of drug resistance against all currently used anti-HIV-1 molecules necessitates the development of new antiviral molecules and\/or strategies against HIV-1 infection. In this study, we have identified a benzamide derivative named AH0109 that exhibits potent anti-HIV-1 activity at an 50% effective concentration of 0.7 \u03bcM in HIV-1-susceptible CD4\n            <jats:sup>+<\/jats:sup>\n            C8166 T cells. Mechanistic analysis revealed that AH0109 significantly inhibits both HIV-1 reverse transcription and viral cDNA nuclear import. Furthermore, our infection experiments indicated that AH0109 is capable of disrupting the replication of HIV-1 strains that are resistant to the routinely used anti-HIV-1 drugs zidovudine, lamivudine, nevirapine, and raltegravir. Together, these findings provide evidence for a newly identified antiviral molecule that can potentially be developed as an anti-HIV-1 agent.\n          <\/jats:p>","DOI":"10.1128\/aac.00100-13","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T04:58:27Z","timestamp":1368507507000},"page":"3547-3554","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Characterization of Antiviral Activity of Benzamide Derivative AH0109 against HIV-1 Infection"],"prefix":"10.1128","volume":"57","author":[{"given":"Liyu","family":"Chen","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"},{"name":"Department of Microbiology, School of Basic Medical Sciences, Central South University, Changsha, Hunan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhujun","family":"Ao","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kallesh Danappa","family":"Jayappa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Kobinger","sequence":"additional","affiliation":[{"name":"Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ShuiPing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Basic Medical Sciences, Central South University, Changsha, Hunan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guojun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Basic Medical Sciences, Central South University, Changsha, Hunan, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Wainberg","sequence":"additional","affiliation":[{"name":"Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University AIDS Centre, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojian","family":"Yao","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00250"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.33.040193.001053"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir728"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb17823.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568026043388547"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-2-62"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.18.9825"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00508-10"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303594200"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2009.09.009"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.7.12.1541"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.10.010"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R112.389056"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0b013e3283567309"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/v4020309"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2010.012"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001995"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.184739"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01164-10"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.7.1386"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.04.011"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-11-112"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05578-11"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.17.8033"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.11.7032-7044.1995"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2009.04.020"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60597-2"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.24.11241"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/v2040880"},{"key":"e_1_3_2_31_2","first-page":"70","article-title":"The HIV-1 passage from cytoplasm to nucleus: the process involving a complex exchange between the components of HIV-1 and cellular machinery to access nucleus and successful integration","volume":"3","author":"Jayappa KD","year":"2012","unstructured":"JayappaKD AoZ YaoX. 2012. The HIV-1 passage from cytoplasm to nucleus: the process involving a complex exchange between the components of HIV-1 and cellular machinery to access nucleus and successful integration. Int. J. Biochem. Mol. Biol. 3:70\u201385.","journal-title":"Int. J. Biochem. Mol. Biol"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11859"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1086\/644505"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e3283388742"},{"key":"e_1_3_2_35_2","first-page":"62","article-title":"Update of the drug resistance mutations in HIV-1: Spring 2008","volume":"16","author":"Johnson VA","year":"2008","unstructured":"JohnsonVA Brun-VezinetF ClotetB GunthardHF KuritzkesDR PillayD SchapiroJM RichmanDD. 2008. Update of the drug resistance mutations in HIV-1: Spring 2008. Top. HIV Med. 16:62\u201368.","journal-title":"Top. HIV Med"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00100-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00100-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:32:44Z","timestamp":1645482764000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00100-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1128\/AAC.00100-13"],"URL":"https:\/\/doi.org\/10.1128\/aac.00100-13","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8]]},"assertion":[{"value":"2013-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}