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The objectives of our work were to produce and purify YEM-1 and to complete its kinetic characterization. YEM-1 displayed the narrowest substrate range among known subclass B2 metallo-\u03b2-lactamases, since it can hydrolyze imipenem, but not other carbapenems, such as biapenem, meropenem, doripenem, and ertapenem, with high catalytic efficiency.\n                  <\/jats:p>","DOI":"10.1128\/aac.00105-20","type":"journal-article","created":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T10:11:20Z","timestamp":1591870280000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Mutational Effects on Carbapenem Hydrolysis of YEM-1, a New Subclass B2 Metallo-\u03b2-Lactamase from Yersinia mollaretii"],"prefix":"10.1128","volume":"64","author":[{"given":"Paola Sandra","family":"Mercuri","sequence":"first","affiliation":[{"name":"Macromol\u00e9cules Biologiques, Centre d\u2019Ing\u00e9nierie des Prot\u00e9ines, InBioS, Universit\u00e9 de Li\u00e8ge, Li\u00e8ge, 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