{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:21:04Z","timestamp":1760782864600},"reference-count":32,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            When tested against 254\n            <jats:italic>Haemophilus influenzae<\/jats:italic>\n            strains, LBM415, a peptide deformylase inhibitor, gave MIC\n            <jats:sub>50<\/jats:sub>\n            and MIC\n            <jats:sub>90<\/jats:sub>\n            values of 2.0 \u03bcg\/ml and 8.0 \u03bcg\/ml, respectively. The MICs were independent of \u03b2-lactam or quinolone susceptibility and the presence or absence of macrolide efflux or ribosomal protein mutations. The MICs of LBM415 against 23\n            <jats:italic>H. parainfluenzae<\/jats:italic>\n            strains were similar to those against\n            <jats:italic>H. influenzae<\/jats:italic>\n            . In contrast, erythromycin, azithromycin, and clarithromycin gave unimodal MIC distributions, and apart from \u03b2-lactamase-negative, ampicillin-resistant strains, all strains were susceptible to the \u03b2-lactams tested. Apart from selected quinolone-resistant strains, all strains were susceptible to ciprofloxacin, levofloxacin, gatifloxacin, moxifloxacin, and gemifloxacin. Resistance to trimethoprim-sulfamethoxazole was common. The potencies of all drugs against 23\n            <jats:italic>H. parainfluenzae<\/jats:italic>\n            strains were similar to those against\n            <jats:italic>H. influenzae<\/jats:italic>\n            . Time-kill studies with 10\n            <jats:italic>Haemophilus<\/jats:italic>\n            strains showed LBM415 to be bactericidal at 2\u00d7 the MIC against 8 of 10 strains after 24 h. For comparison, the macrolides and \u03b2-lactams were bactericidal against 8 to 10 strains each at 2\u00d7 the MIC after 24 h. Quinolones were bactericidal against all 10 strains tested at 2\u00d7 the MIC after 24 h. Against six\n            <jats:italic>H. influenzae<\/jats:italic>\n            strains, postantibiotic effects for LBM415 lasted between 0.8 and 2.2 h. In multistep resistance selection studies, LBM415 produced resistant clones in 7 of the 10 strains tested, with MICs ranging from 4 to 64 \u03bcg\/ml. No mutations in deformylase (\n            <jats:italic>def<\/jats:italic>\n            ) and formyltransferase (\n            <jats:italic>fmt<\/jats:italic>\n            ) genes were detected in any of the LBM415-resistant mutants.\n          <\/jats:p>","DOI":"10.1128\/aac.00106-06","type":"journal-article","created":{"date-parts":[[2006,6,26]],"date-time":"2006-06-26T18:26:28Z","timestamp":1151346388000},"page":"2323-2329","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Activity of LBM415 Compared to Those of 11 Other Agents against\n            <i>Haemophilus<\/i>\n            Species"],"prefix":"10.1128","volume":"50","author":[{"given":"Tatiana","family":"Bogdanovich","sequence":"first","affiliation":[{"name":"Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathy A.","family":"Smith","sequence":"additional","affiliation":[{"name":"Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Clark","sequence":"additional","affiliation":[{"name":"Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn A.","family":"Pankuch","sequence":"additional","affiliation":[{"name":"Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gengrong","family":"Lin","sequence":"additional","affiliation":[{"name":"Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"McGhee","sequence":"additional","affiliation":[{"name":"Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bonifacio","family":"Dewasse","sequence":"additional","affiliation":[{"name":"Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C.","family":"Appelbaum","sequence":"additional","affiliation":[{"name":"Hershey Medical Center, Hershey, Pennsylvania 17033"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(03)00114-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0911.030176"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2956-2962.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.563-570.2001"},{"key":"e_1_3_2_6_2","first-page":"265","year":"1996","unstructured":"Craig. 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