{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:17:47Z","timestamp":1791353867241,"version":"4.3.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Integrating conjugative elements (ICEs) are mobile genetic elements that can transfer from the chromosome of a host to the chromosome of a new host through the process of excision, conjugation, and integration. Although SXT\/R391-related ICEs, originally demonstrated in\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            O139 isolates, have become prevalent among\n            <jats:italic>V. cholerae<\/jats:italic>\n            isolates in Asia, the prevalence of the ICEs among Gram-negative bacteria other than\n            <jats:italic>Vibrio<\/jats:italic>\n            spp. remains unknown. In addition, SXT\/R391-related ICEs carrying genes conferring resistance to extended-spectrum cephalosporins have never been described. Here we carried out a genetic analysis of a cefoxitin-resistant\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            clinical isolate, TUM4660, which revealed the presence of a novel SXT\/R391-related ICE, ICE\n            <jats:italic>Pmi<\/jats:italic>\n            Jpn1. ICE\n            <jats:italic>Pmi<\/jats:italic>\n            Jpn1 had a core genetic structure showing high similarity to that of R391 and carried\n            <jats:italic>xis<\/jats:italic>\n            and\n            <jats:italic>int<\/jats:italic>\n            genes completely identical to those of R391, while an IS\n            <jats:italic>10<\/jats:italic>\n            -mediated composite transposon carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            was integrated into the ICE. A nucleotide sequence identical to the 3\u2032 part of IS\n            <jats:italic>Ecp1<\/jats:italic>\n            was located upstream of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            gene, and other genes observed around\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            in earlier studies were also present. Furthermore, the nucleotide sequences of hot spot 2 and hot spot 4 in ICE\n            <jats:italic>Pmi<\/jats:italic>\n            Jpn1 showed high similarity to that of hot spot 2 in SXT\n            <jats:sup>MO10<\/jats:sup>\n            and with a part of the nucleotide sequence found in\n            <jats:italic>P. mirabilis<\/jats:italic>\n            ATCC 29906, respectively. ICE\n            <jats:italic>Pmi<\/jats:italic>\n            Jpn1 was successfully transferred to\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ,\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            ,\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Typhimurium, and\n            <jats:italic>Citrobacter koseri<\/jats:italic>\n            in conjugation experiments. These observations suggest that ICEs may contribute to the dissemination of antimicrobial resistance genes among clinically relevant\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            , which warrants careful observation of the prevalence of ICEs, including SXT\/R391-related ICEs.\n          <\/jats:p>","DOI":"10.1128\/aac.00111-10","type":"journal-article","created":{"date-parts":[[2010,6,22]],"date-time":"2010-06-22T02:41:31Z","timestamp":1277174491000},"page":"3545-3550","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Chromosomally Encoded\n            <i>bla<\/i>\n            <sub>CMY-2<\/sub>\n            Located on a Novel SXT\/R391-Related Integrating Conjugative Element in a\n            <i>Proteus mirabilis<\/i>\n            Clinical Isolate"],"prefix":"10.1128","volume":"54","author":[{"given":"Sohei","family":"Harada","sequence":"first","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, School of Medicine, Faculty of Medicine, Toho University, Tokyo"},{"name":"Department of Infectious Diseases, Graduate School of Medicine, University of Tokyo, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, School of Medicine, Faculty of Medicine, Toho University, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoo","family":"Saga","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, School of Medicine, Faculty of Medicine, Toho University, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Tateda","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, School of Medicine, Faculty of Medicine, Toho University, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keizo","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, School of Medicine, Faculty of Medicine, Toho University, Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.11.012"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0904.020317"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000180200006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.15.4259-4269.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.18.5158-5169.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.5.1110"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2006.01.001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.3054-3057.2006"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.9.2636-2645.2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.5.1269-1272.2002"},{"key":"e_1_3_2_12_2","unstructured":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; 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