{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:10:45Z","timestamp":1791292245345,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The contribution of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            gene and its promoter to \u03b2-lactam resistance has not been validated in\n            <jats:italic>Acinetobacter<\/jats:italic>\n            spp. other than\n            <jats:italic>Acinetobacter baumannii<\/jats:italic>\n            . We identified a multidrug-resistant (including carbapenem resistance)\n            <jats:italic>Acinetobacter<\/jats:italic>\n            genomic species 13TU in which\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            was the only detected carbapenemase gene. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            gene was plasmid located, flanked by IS\n            <jats:italic>Aba3<\/jats:italic>\n            (downstream) and an IS\n            <jats:italic>Aba3-<\/jats:italic>\n            like element (upstream). An IS\n            <jats:italic>1006<\/jats:italic>\n            element was inserted into IS\n            <jats:italic>Aba3<\/jats:italic>\n            -like (IS\n            <jats:italic>1006<\/jats:italic>\n            -\u0394IS\n            <jats:italic>Aba3<\/jats:italic>\n            -like) to generate a hybrid promoter for\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            , with a \u221235 promoter located in IS\n            <jats:italic>1006<\/jats:italic>\n            and a \u221210 promoter in IS\n            <jats:italic>Aba3<\/jats:italic>\n            -like. The reference strain of\n            <jats:italic>Acinetobacter<\/jats:italic>\n            genomic species 13TU, ATCC 17903, revealed higher MICs of amoxicillin, ticarcillin, and piperacillin and heteroresistance to imipenem and meropenem when it was transformed with a shuttle vector containing a fragment encompassing \u0394IS\n            <jats:italic>Aba3<\/jats:italic>\n            -like-\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            , compared to the same host containing only\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            . When the fragment was changed from \u0394IS\n            <jats:italic>Aba3<\/jats:italic>\n            -like-\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            to IS\n            <jats:italic>1006<\/jats:italic>\n            -\u0394IS\n            <jats:italic>Aba3<\/jats:italic>\n            -like-\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            , the ATCC 17903 transformant revealed a markedly higher level of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            transcription (12-fold), increased cefuroxime and piperacillin-tazobactam MICs, and homoresistance to imipenem and meropenem. Different roles of the insertion elements preceding the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            gene in\n            <jats:italic>Acinetobacter<\/jats:italic>\n            genomic species 13TU are demonstrated. The IS\n            <jats:italic>Aba3<\/jats:italic>\n            -like-\n            <jats:italic>-bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            construct can mediate resistance to penicillin derivatives but only heteroresistance to carbapenems. The insertion of IS\n            <jats:italic>1006<\/jats:italic>\n            into IS\n            <jats:italic>Aba3<\/jats:italic>\n            -like, generating a hybrid promoter, could further enhance the transcription of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            and mediate homoresistance to carbapenems and also enhanced resistance to piperacillin-tazobactam.\n          <\/jats:p>","DOI":"10.1128\/aac.00128-10","type":"journal-article","created":{"date-parts":[[2010,6,2]],"date-time":"2010-06-02T01:10:43Z","timestamp":1275441043000},"page":"3107-3112","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Contribution of a Plasmid-Borne\n            <i>bla<\/i>\n            <sub>OXA-58<\/sub>\n            Gene with Its Hybrid Promoter Provided by IS\n            <i>1006<\/i>\n            and an IS\n            <i>Aba3<\/i>\n            -Like Element to \u03b2-Lactam Resistance in\n            <i>Acinetobacter<\/i>\n            Genomic Species 13TU"],"prefix":"10.1128","volume":"54","author":[{"given":"Te-Li","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei"},{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Che","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Chen","family":"Kuo","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Tzu","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medicine, Chutung Veterans Hospital, Chutung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Pei","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leung-Kei","family":"Siu","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, National Health Research Institute, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Long","family":"Cho","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei"},{"name":"Mackay Medical College, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Phone","family":"Fung","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei"},{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01502-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.1632-1639.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00393-08"},{"key":"e_1_3_2_5_2","unstructured":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; 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