{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:59:31Z","timestamp":1792004371867,"version":"4.4.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Pfizer's Centers for Therapeutic Innovation","award":["24-PZR-09"],"award-info":[{"award-number":["24-PZR-09"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      We evaluated the presence and phenotypic impact of PBP3 insertions YRIN (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 44) and YRIK (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 18) among 124\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      isolates belonging to high-risk lineages selected from 1,471 isolates displaying resistance to \u03b2-lactams. PBP3 insertions were detected among 62 isolates. YRIN insertions were detected among isolates belonging to ST167 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 23), ST361 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 7), ST410 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 10), ST617 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 1), and ST648 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 3), while YRIK insertions were observed in ST405 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 9), ST410 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 5), and ST617 (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 4). Aztreonam-avibactam inhibited 90.3% of all isolates from high-risk clones and 80.6% of the isolates harboring PBP3 insertions when applying the EUCAST breakpoints. Aztreonam-avibactam inhibited 66.7% of the YRIK-harboring isolates (MIC\n                      <jats:sub>50\/90<\/jats:sub>\n                      , 2\/16 mg\/L) and 86.4% of the ones harboring YRIN insertions (MIC\n                      <jats:sub>50\/90<\/jats:sub>\n                      , 1\/8 mg\/L); however, the susceptibilities to other \u03b2-lactams were lower among YRIN isolates (25.6%\u201360.5%) compared to YRIK (55.6%\u201388.9%). Overall, ST617 was more resistant to all agents when compared to other STs, regardless of PBP3 insertions or the presence of other examined resistance mechanisms. Acquired \u03b2-lactamase genes and OmpC deleterious alterations were present in all isolates. CMY-42, CMY-141, and CMY-145 that have been associated with elevated aztreonam-avibactam MICs were detected among 13 isolates, and 4 (30.8%) were susceptible to this combination. All isolates harboring\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM-5<\/jats:sub>\n                      (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 37) possessed PBP3 insertions. Aztreonam-avibactam inhibited 83.8% of these isolates. Resistance to aztreonam-avibactam is multifactorial, and singular resistance mechanisms are unable to elevate MIC values to resistant categories. Additionally, lineage-specific contributions to resistance play an important role in the overall development of \u03b2-lactam resistance, although these mechanisms appear to function outside the known repertoire of resistance-conferring mutations and warrant further study.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00129-26","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:38:56Z","timestamp":1788964736000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Impact of PBP3 insertions and \u03b2-lactamase-encoding genes on the susceptibility of\n                    <i>Escherichia coli<\/i>\n                    isolates belonging to high-risk clones against aztreonam-avibactam and comparator agents"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0126-1782","authenticated-orcid":true,"given":"Mariana","family":"Castanheira","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02qv6pw23","id-type":"ROR","asserted-by":"publisher"}],"name":"Element Materials Technology (JMI Laboratories)","place":["North Liberty, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0457-1543","authenticated-orcid":false,"given":"John H.","family":"Kimbrough","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02qv6pw23","id-type":"ROR","asserted-by":"publisher"}],"name":"Element Materials Technology (JMI Laboratories)","place":["North Liberty, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8759-1290","authenticated-orcid":false,"given":"Maura","family":"Karr","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02qv6pw23","id-type":"ROR","asserted-by":"publisher"}],"name":"Element Materials Technology (JMI Laboratories)","place":["North Liberty, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7022-6644","authenticated-orcid":true,"given":"Helio S.","family":"Sader","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02qv6pw23","id-type":"ROR","asserted-by":"publisher"}],"name":"Element Materials Technology (JMI Laboratories)","place":["North Liberty, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000238720"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.21.6.950"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlaf131"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10081012"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab279"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01548-23"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-024-04769-z"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01659-20"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlae141"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2024.107256"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2024.107113"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofaf005"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-023-04645-2"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00842-25"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofaf250"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlad032"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkae393"},{"key":"e_1_3_3_20_2","volume-title":"M07Ed12","author":"CLSI","year":"2024","unstructured":"CLSI. 2024. 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