{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:00:09Z","timestamp":1791518409320,"version":"4.3.4"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T00:00:00Z","timestamp":1532649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000268","name":"RCUK | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/J006637\/1"],"award-info":[{"award-number":["BB\/J006637\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"RCUK | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/J009725\/1"],"award-info":[{"award-number":["BB\/J009725\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The tunicamycin biosynthetic gene cluster of\n            <jats:named-content content-type=\"genus-species\">Streptomyces chartreusis<\/jats:named-content>\n            consists of 14 genes (\n            <jats:italic>tunA<\/jats:italic>\n            to\n            <jats:italic>tunN<\/jats:italic>\n            ) with a high degree of apparent translational coupling. Transcriptional analysis revealed that all of these genes are likely to be transcribed as a single operon from two promoters,\n            <jats:italic>tun<\/jats:italic>\n            p1 and\n            <jats:italic>tun<\/jats:italic>\n            p2. In-frame deletion analysis revealed that just six of these genes (\n            <jats:italic>tunABCDEH<\/jats:italic>\n            ) are essential for tunicamycin production in the heterologous host\n            <jats:named-content content-type=\"genus-species\">Streptomyces coelicolor<\/jats:named-content>\n            , while five (\n            <jats:italic>tunFGKLN<\/jats:italic>\n            ) with likely counterparts in primary metabolism are not necessary, but presumably ensure efficient production of the antibiotic at the onset of tunicamycin biosynthesis. Three genes are implicated in immunity, namely,\n            <jats:italic>tunI<\/jats:italic>\n            and\n            <jats:italic>tunJ<\/jats:italic>\n            , which encode a two-component ABC transporter presumably required for export of the antibiotic, and\n            <jats:italic>tunM<\/jats:italic>\n            , which encodes a putative\n            <jats:italic>S<\/jats:italic>\n            -adenosylmethionine (SAM)-dependent methyltransferase. Expression of\n            <jats:italic>tunIJ<\/jats:italic>\n            or\n            <jats:italic>tunM<\/jats:italic>\n            in\n            <jats:named-content content-type=\"genus-species\">S. coelicolor<\/jats:named-content>\n            conferred resistance to exogenous tunicamycin. The results presented here provide new insights into tunicamycin biosynthesis and immunity.\n          <\/jats:p>","DOI":"10.1128\/aac.00130-18","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T10:12:22Z","timestamp":1527502342000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Analysis of the Tunicamycin Biosynthetic Gene Cluster of Streptomyces chartreusis Reveals New Insights into Tunicamycin Production and Immunity"],"prefix":"10.1128","volume":"62","author":[{"given":"David","family":"Widdick","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain F.","family":"Royer","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia M.","family":"Vior","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Pablo","family":"Gomez-Escribano","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin G.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mervyn J.","family":"Bibb","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.24.215"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06262-11"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.7.1640"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/156802605774463042"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005585"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00578a008"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0sc00325e"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1351"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00434-11"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.18.5854-5861.1997"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201604304"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-010-0127-6"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0230516100"},{"key":"e_1_3_3_15_2","volume-title":"Recombineering in Streptomyces coelicolor","author":"Gust B","year":"2003","unstructured":"Gust B, O'Rourke S, Bird N, Kieser T, Chater KF. 2003. 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