{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:51:54Z","timestamp":1791219114313,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"BIOASTER","award":["BA220"],"award-info":[{"award-number":["BA220"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,6,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      This study comprehensively assessed the activity of manogepix (MNGX), ibrexafungerp (IBF), amphotericin B (AMB), rezafungin (RZF), and caspofungin (CAS) against planktonic cells and mature biofilms of\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      spp.\u2014reference and clinical strains using the Calgary biofilm device. Mature-phase biofilms of\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">C. auris (clades I, II, III, IV<\/jats:italic>\n                      ), and\n                      <jats:italic toggle=\"yes\">C. parapsilosis<\/jats:italic>\n                      were exposed to a range of drug concentrations (0.12\u2013128 \u00b5g\/mL). Minimum Inhibitory Concentration (MIC) values for planktonic cells were \u22642 \u00b5g\/mL for all strains; however, biofilm-associated MICs, minimum biocidal concentration (MBC), minimum biofilm eradication (MBEC), and minimum biofilm damaging concentration (MBDC) were significantly higher (2\u20134,119 times). Geometric mean (GM) of MBEC values indicated that MNGX had the highest antifungal activity within\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      species, with a GM-MBEC of 5.9 \u00b5g\/mL. Despite its overall potency, MNGX was less effective against\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      biofilms from clade IV strains, where IBF showed superior activity. While not the most potent agent overall, AMB induced the smallest fold-change increases (2- to 32-fold) in biofilm-associated states data compared to planktonic MICs. Conversely, CAS exhibited the lowest activity against\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      spp. biofilms. The eradication of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">C. parapsilosis<\/jats:italic>\n                      biofilms required substantially higher concentrations than\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      , with some agents, such as RZF and CAS, necessitating up to 42-fold increases in dosage. In conclusion, our\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      model highlights the antibiofilm activity of novel antifungals against major\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      species, revealing significant differences in efficacy among species. MNGX demonstrated the highest activity, underscoring its potential as a promising candidate for the treatment of biofilm-related infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00137-25","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T09:00:21Z","timestamp":1747299621000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Antibiofilm activity of manogepix, ibrexafungerp, amphotericin B, rezafungin, and caspofungin against\n                    <i>Candida<\/i>\n                    spp. biofilms of reference and clinical strains"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2129-1399","authenticated-orcid":false,"given":"Andres","family":"Ceballos-Garzon","sequence":"first","affiliation":[{"name":"BIOASTER Research Institute","place":["Lyon, France"]},{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad del Rosario","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Lebrat","sequence":"additional","affiliation":[{"name":"BIOASTER Research Institute","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Holzapfel","sequence":"additional","affiliation":[{"name":"BIOASTER Research Institute","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego F.","family":"Josa","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Universidad de La Sabana","place":["Bogota, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Welsch","sequence":"additional","affiliation":[{"name":"BIOASTER Research Institute","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derry","family":"Mercer","sequence":"additional","affiliation":[{"name":"BIOASTER Research Institute","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2415"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.13.101"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2019.04.001"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2006.00117.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40588-020-00143-7"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3934\/microbiol.2023025"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0263522"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics4010001"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00749-7"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00028"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.107"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof9100955"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-53588-5"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphereDirect.00680-18"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-021-01611-0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof7030163"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx010"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00731-11"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01750-17"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2006.apm_394.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.2174\/1874437001105010009"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1771-1776.1999"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1251\/bpo127"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1001.030119"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0702.010226"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2302.161320"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05072.x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00498-23"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyab029"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01508-24"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13223"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.530032"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.6.1773-1780.2002"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02396-16"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab454"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz565"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03117-14"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00959-10"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03065-15"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2016.01.007"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000828"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21228873"},{"key":"e_1_3_3_44_2","volume-title":"M27Reference method for broth dilution antifungal susceptibility testing of yeasts","author":"Anon","year":"2017","unstructured":"Anon, Clinical and Laboratory Standards Institute. 2017. M27. Reference method for broth dilution antifungal susceptibility testing of yeasts. 4th ed. CLSI, Wayne, PA, USA.","edition":"4"},{"key":"e_1_3_3_45_2","volume-title":"CLSI supplement M27M44SPerformance standards for antifungal susceptibility testing of yeast","author":"CLSI","year":"2022","unstructured":"CLSI. 2022. CLSI supplement M27M44S. Performance standards for antifungal susceptibility testing of yeast. 3rd ed. Clinical and Laboratory standars institute.","edition":"3"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlaf040"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0265777"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252312720"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9118-1_6"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701581458"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof7070574"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00137-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00137-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T09:01:11Z","timestamp":1749027671000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.00137-25"}},"subtitle":[],"editor":[{"given":"Andreas H.","family":"Groll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,6,4]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6,4]]}},"alternative-id":["10.1128\/aac.00137-25"],"URL":"https:\/\/doi.org\/10.1128\/aac.00137-25","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,4]]},"assertion":[{"value":"2025-01-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00137-25"}}