{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T10:28:34Z","timestamp":1740133714310,"version":"3.37.3"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T00:00:00Z","timestamp":1466380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Artemisinin resistance in\n Plasmodium falciparum<\/jats:named-content>\n , the agent of severe malaria, is currently a major obstacle to malaria control in Southeast Asia. A gene named \u201c\n kelch13<\/jats:italic>\n \u201d has been associated with artemisinin resistance in\n P. falciparum<\/jats:named-content>\n . The orthologue of the\n kelch<\/jats:italic>\n gene in\n P. vivax<\/jats:named-content>\n was identified and a small number of mutations were found in previous studies. The\n kelch<\/jats:italic>\n orthologues in the other two human malaria parasites,\n P. malariae<\/jats:named-content>\n and\n P. ovale<\/jats:named-content>\n , have not yet been studied. Therefore, in this study, the orthologous\n kelch<\/jats:italic>\n genes of\n P. malariae<\/jats:named-content>\n ,\n P. ovale wallikeri<\/jats:named-content>\n , and\n P. ovale curtisi<\/jats:named-content>\n were isolated and analyzed for the first time. The homologies of the\n kelch<\/jats:italic>\n genes of\n P. malariae<\/jats:named-content>\n and\n P. ovale<\/jats:named-content>\n were 84.8% and 82.7%, respectively, compared to the gene in\n P. falciparum<\/jats:named-content>\n .\n kelch<\/jats:italic>\n polymorphisms were studied in 13\n P. malariae<\/jats:italic>\n and 5\n P. ovale<\/jats:named-content>\n isolates from Thailand. There were 2 nonsynonymous mutations found in these samples. One mutation was P533L, which was found in 1 of 13\n P. malariae<\/jats:named-content>\n isolates, and the other was K137R, found in 1 isolate of\n P. ovale wallikeri<\/jats:named-content>\n (\n n<\/jats:italic>\n = 4). This result needs to be considered in the context of widespread artemisinin used within the region; their functional consequences for artemisinin sensitivity in\n P. malariae<\/jats:named-content>\n and\n P. ovale<\/jats:named-content>\n will need to be elucidated.\n <\/jats:p>","DOI":"10.1128\/aac.00138-16","type":"journal-article","created":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T04:08:19Z","timestamp":1461643699000},"page":"4055-4062","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Limited Polymorphism of the Kelch Propeller Domain in Plasmodium malariae and P. ovale Isolates from Thailand"],"prefix":"10.1128","volume":"60","author":[{"given":"Supatchara","family":"Nakeesathit","sequence":"first","affiliation":[{"name":"Mahidol Oxford Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naowarat","family":"Saralamba","sequence":"additional","affiliation":[{"name":"Mahidol Oxford Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sasithon","family":"Pukrittayakamee","sequence":"additional","affiliation":[{"name":"Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjen","family":"Dondorp","sequence":"additional","affiliation":[{"name":"Mahidol Oxford Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Centre for Tropical Medicine, Nuffield Department of Medicine, Churchill Hospital, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francois","family":"Nosten","sequence":"additional","affiliation":[{"name":"Centre for Tropical Medicine, Nuffield Department of Medicine, Churchill Hospital, Oxford, United Kingdom"},{"name":"Shoklo Malaria Research Unit, Tak, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"White","sequence":"additional","affiliation":[{"name":"Mahidol Oxford Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Centre for Tropical Medicine, Nuffield Department of Medicine, Churchill Hospital, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mallika","family":"Imwong","sequence":"additional","affiliation":[{"name":"Mahidol Oxford Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"WHO malaria report 2014","author":"WHO","year":"2014","unstructured":"WHO. 2014. WHO malaria report 2014. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006001958"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/652240"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.3.570-581.2005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12876"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1314981"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03908-14"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1996.tb04497.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136099"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01527-13"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)70032-0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3189"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu397"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.08714"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093942"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.09-0022"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00138-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00138-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:35:54Z","timestamp":1645475754000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00138-16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":17,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1128\/AAC.00138-16"],"URL":"https:\/\/doi.org\/10.1128\/aac.00138-16","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"type":"print","value":"0066-4804"},{"type":"electronic","value":"1098-6596"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"2016-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}