{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:46:36Z","timestamp":1785851196434,"version":"3.56.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Brazilian National Council for Scientific and Technological Development-CNPq","award":["164367\/2021-7"],"award-info":[{"award-number":["164367\/2021-7"]}]},{"name":"Brazilian National Council for Scientific and Technological Development-CNPq","award":["140791\/2022-1"],"award-info":[{"award-number":["140791\/2022-1"]}]},{"name":"Brazilian National Council for Scientific and Technological Development-CNPq","award":["307891\/2025-9"],"award-info":[{"award-number":["307891\/2025-9"]}]},{"name":"Brazilian National Council for Scientific and Technological Development-CNPq","award":["310445\/2017-5"],"award-info":[{"award-number":["310445\/2017-5"]}]},{"name":"Brazilian National Council for Scientific and Technological Development-CNPq","award":["316462\/2021-7"],"award-info":[{"award-number":["316462\/2021-7"]}]},{"name":"National Institute of Science and Technology in Neuroimmunomodulation","award":["408201\/2024-0"],"award-info":[{"award-number":["408201\/2024-0"]}]},{"name":"Rio de Janeiro research funding agency","award":["E-26\/200.314\/2025"],"award-info":[{"award-number":["E-26\/200.314\/2025"]}]},{"name":"Rio de Janeiro research funding agency","award":["E-26\/200.491\/2025"],"award-info":[{"award-number":["E-26\/200.491\/2025"]}]},{"name":"Rio de Janeiro research funding agency","award":["E-26\/200.492\/2025"],"award-info":[{"award-number":["E-26\/200.492\/2025"]}]},{"name":"Rio de Janeiro research funding agency","award":["E-26\/202.921\/2018"],"award-info":[{"award-number":["E-26\/202.921\/2018"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Artemether and artesunate are effective antimalarial drugs in rescue therapies of\n                      <jats:italic toggle=\"yes\">Plasmodium berghei<\/jats:italic>\n                      ANKA (PbA)-infected mice with experimental cerebral malaria (ECM), with a superiority for artemether among the single daily dosing schemes. The present study aimed to optimize the efficacy of artesunate treatment protocols using two daily dosing or slow continuous delivery schemes. PbA-infected mice were treated with four different artesunate protocols on day 5 post-infection (5 dpi). Protocol G4 (single intraperitoneal (IP) loading dose of 20 mg\/kg followed by continuous delivery for 3 days with osmotic pumps) was superior to the other artesunate groups, with a 97.2% \u00b1 0.86 reduction of parasitemia in 24 h, but recrudescence ensued in 50% of treated animals. Protocol G1 (loading dose of 40 mg\/kg followed by 20 mg\/kg twice a day for 5 days) led to a 90.5% \u00b15.39 decrease in parasitemia 24 h after the first dose without recrudescence. A variant of the G1 protocol, with two doses of 40 mg\/kg in the first day, resulted in a survival rate of 42% in ECM-rescuing experiments, with ~30% decreases in hematocrit, hemoglobin, and red blood cell count 24 h after the first dose. Infected mice on 5 dpi showed severe thrombocytopenia (196 \u00b1 118.3 platelets\/\u03bcL; uninfected controls: 1,291 \u00b1 256.4 platelets\/\u03bcL), and artesunate treatment had little impact on these numbers 24 h after the first dose. In conclusion, two daily doses IP or via slow continuous delivery improve the efficacy of artesunate treatments compared to single-daily dose schemes and are suitable for ECM adjunctive therapy studies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00154-26","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:03:20Z","timestamp":1781795000000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Improved protocols using sodium artesunate for the treatment of experimental cerebral malaria"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9431-1590","authenticated-orcid":true,"given":"Melina Pedroso","family":"Merlone","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme Salgado","family":"Sanches","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udio Tadeu","family":"Daniel-Ribeiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0133-6430","authenticated-orcid":true,"given":"Leonardo Jos\u00e9 de Moura","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"WHO: World Health Organization. 2025. 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