{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:55:37Z","timestamp":1790250937235,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,5,24]],"date-time":"2017-05-24T00:00:00Z","timestamp":1495584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001349","name":"MOH | National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\/TCR\/011-NUHS\/2014"],"award-info":[{"award-number":["NMRC\/TCR\/011-NUHS\/2014"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Lung infections caused by\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium abscessus<\/jats:named-content>\n                    are emerging as a global threat to individuals with cystic fibrosis and to other patient groups. Recent evidence for human-to-human transmission worsens the situation.\n                    <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n                    is an intrinsically multidrug-resistant pathogen showing resistance to even standard antituberculosis drugs, such as rifampin. Here, our objective was to identify existing drugs that may be employed for the treatment of\n                    <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n                    lung disease. A collection of more than 2,700 approved drugs was screened at a single-point concentration against an\n                    <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n                    clinical isolate. Hits were confirmed with fresh solids in dose-response experiments. For the most attractive hit, growth inhibition and bactericidal activities against reference strains of the three\n                    <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n                    subspecies and a collection of clinical isolates were determined. Surprisingly, the rifampin derivative rifabutin had MICs of 3 \u00b1 2 \u03bcM (3 \u03bcg\/ml) against the screening strain, the reference strains\n                    <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n                    subsp.\n                    <jats:named-content content-type=\"genus-species\">abscessus<\/jats:named-content>\n                    ATCC 19977,\n                    <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n                    subsp.\n                    <jats:named-content content-type=\"genus-species\">bolletii<\/jats:named-content>\n                    CCUG 50184-T, and\n                    <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n                    subsp.\n                    <jats:named-content content-type=\"genus-species\">massiliense<\/jats:named-content>\n                    CCUG 48898-T, as well as against a collection of clinical isolates. Furthermore, rifabutin was active against clarithromycin-resistant strains. In conclusion, rifabutin, in contrast to rifampin, is active against the\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium abscessus<\/jats:named-content>\n                    complex bacteria\n                    <jats:italic>in vitro<\/jats:italic>\n                    and may be considered for treatment of\n                    <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n                    lung disease.\n                  <\/jats:p>","DOI":"10.1128\/aac.00155-17","type":"journal-article","created":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T20:30:32Z","timestamp":1491856232000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":138,"title":["Rifabutin Is Active against Mycobacterium abscessus Complex"],"prefix":"10.1128","volume":"61","author":[{"given":"Dinah Binte","family":"Aziz","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian Liang","family":"Low","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu-Lu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Gengenbacher","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9321-3341","authenticated-orcid":false,"given":"Jeanette W. 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