{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:07:19Z","timestamp":1791158839321,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The relationship between efflux system overexpression and cross-resistance to cefoxitin, quinolones, and chloramphenicol has recently been reported in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            . In 3 previously published clinical isolates and 17\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">in vitro<\/jats:named-content>\n            mutants selected with cefoxitin or fluoroquinolones, mutations in the potential regulator genes of the AcrAB efflux pump (\n            <jats:italic>acrR<\/jats:italic>\n            ,\n            <jats:italic>ramR<\/jats:italic>\n            ,\n            <jats:italic>ramA<\/jats:italic>\n            ,\n            <jats:italic>marR<\/jats:italic>\n            ,\n            <jats:italic>marA<\/jats:italic>\n            ,\n            <jats:italic>soxR<\/jats:italic>\n            ,\n            <jats:italic>soxS<\/jats:italic>\n            , and\n            <jats:italic>rob<\/jats:italic>\n            ) were searched, and their impacts on efflux-related antibiotic cross-resistance were assessed. All mutants but 1, and 2 clinical isolates, overexpressed\n            <jats:italic>acrB<\/jats:italic>\n            . No mutation was detected in the regulator genes studied among the clinical isolates and 8 of the mutants. For the 9 remaining mutants, a mutation was found in the\n            <jats:italic>ramR<\/jats:italic>\n            gene in 8 of them and in the\n            <jats:italic>soxR<\/jats:italic>\n            gene in the last one, resulting in overexpression of\n            <jats:italic>ramA<\/jats:italic>\n            and\n            <jats:italic>soxS<\/jats:italic>\n            , respectively. Transformation of the\n            <jats:italic>ramR<\/jats:italic>\n            mutants and the\n            <jats:italic>soxR<\/jats:italic>\n            mutant with the wild-type\n            <jats:italic>ramR<\/jats:italic>\n            and\n            <jats:italic>soxR<\/jats:italic>\n            genes, respectively, abolished overexpression of\n            <jats:italic>acrB<\/jats:italic>\n            and\n            <jats:italic>ramA<\/jats:italic>\n            in the\n            <jats:italic>ramR<\/jats:italic>\n            mutants and of\n            <jats:italic>soxS<\/jats:italic>\n            in the\n            <jats:italic>soxR<\/jats:italic>\n            mutant, as well as antibiotic cross-resistance. Resistance due to efflux system overexpression was demonstrated for 4 new antibiotics: cefuroxime, cefotaxime, ceftazidime, and ertapenem. This study shows that the\n            <jats:italic>ramR<\/jats:italic>\n            and\n            <jats:italic>soxR<\/jats:italic>\n            genes control the expression of efflux systems in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">K. pneumoniae<\/jats:named-content>\n            and suggests the existence of efflux pumps other than AcrAB and of other loci involved in the regulation of AcrAB expression.\n          <\/jats:p>","DOI":"10.1128\/aac.00156-11","type":"journal-article","created":{"date-parts":[[2011,4,5]],"date-time":"2011-04-05T03:18:51Z","timestamp":1301973531000},"page":"2795-2802","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["In Vitro Selection of\n            <i>ramR<\/i>\n            and\n            <i>soxR<\/i>\n            Mutants Overexpressing Efflux Systems by Fluoroquinolones as Well as Cefoxitin in Klebsiella pneumoniae"],"prefix":"10.1128","volume":"55","author":[{"given":"Suzanne","family":"Bialek-Davenet","sequence":"first","affiliation":[{"name":"Service de Microbiologie, H\u00f4pital AP-HP Beaujon, Clichy, France"},{"name":"Facult\u00e9 de M\u00e9decine D. Diderot, Paris, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, U773, Centre de Recherche Biom\u00e9dicale Bichat-Beaujon (CRB3), Universit\u00e9 Paris 7, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Marcon","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, H\u00f4pital AP-HP Beaujon, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Leflon-Guibout","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, H\u00f4pital AP-HP Beaujon, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Lavigne","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Espri 26, Universit\u00e9 Montpellier 1, UFR de M\u00e9decine, N\u00eemes, France"},{"name":"Laboratoire de Bact\u00e9riologie, CHU Caremeau, N\u00eemes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Bert","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, H\u00f4pital AP-HP Beaujon, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Moreau","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, U773, Centre de Recherche Biom\u00e9dicale Bichat-Beaujon (CRB3), Universit\u00e9 Paris 7, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-H\u00e9l\u00e8ne","family":"Nicolas-Chanoine","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, H\u00f4pital AP-HP Beaujon, Clichy, France"},{"name":"Facult\u00e9 de M\u00e9decine D. Diderot, Paris, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, U773, Centre de Recherche Biom\u00e9dicale Bichat-Beaujon (CRB3), Universit\u00e9 Paris 7, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00084-08"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.10.2067"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01607-09"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.8.1469"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp186"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00671.x"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/107662901750152783"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2518-2523.2004"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.5.1484-1492.1993"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp029"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000141"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-141-8-1909"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2701-2706.2004"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00085-10"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00758-07"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.867"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn296"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2007.06.019"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.38-43.2001"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01860.x"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3984-3986.2002"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf044"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.10.004"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.8.2089"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.28.1505"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.1.306-308.1996"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01005-08"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00715-09"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004817"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.11.2200"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00072-07"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.19.2.382-402.2006"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3118-3121.2000"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.4.589"},{"key":"e_1_3_1_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.8.2640-2643.2002"},{"key":"e_1_3_1_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.791-793.2005"},{"key":"e_1_3_1_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.3.1017-1022.2005"},{"key":"e_1_3_1_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2831-2837.2003"},{"key":"e_1_3_1_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/20.3.323"},{"key":"e_1_3_1_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.6.1857-1866.1997"},{"key":"e_1_3_1_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.2.665-669.2003"},{"key":"e_1_3_1_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.10.4390-4392.2005"},{"key":"e_1_3_1_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.17.10323"},{"key":"e_1_3_1_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00315-09"},{"key":"e_1_3_1_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1151-1161.2001"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00156-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00156-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:35:44Z","timestamp":1645486544000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00156-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,6]]}},"alternative-id":["10.1128\/AAC.00156-11"],"URL":"https:\/\/doi.org\/10.1128\/aac.00156-11","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,6]]},"assertion":[{"value":"2011-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-03-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-05-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}